Role of paxillin in the early phase of orientation of the vascular endothelial cells exposed to cyclic stretching

Wenjing Huang, Naoya Sakamoto, Ryotaro Miyazawa, Masaaki Sato

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

Paxillin, a structural and signaling scaffold molecule in focal adhesions (FAs), is considered to be important in intracellular signaling transduction and the cell shape changes in response to cyclic stretching. However, the detailed role of paxillin in stretch-induced morphological changes of endothelial cells (ECs) has not fully determined until date. In this study, in order to understand the role of paxillin in the orientation of ECs exposed to cyclic stretching, we examined the time course of changes in the shape and distribution of FA proteins of paxillin knockdown ECs. Non-treated ECs subjected to 20% cyclic stretching at 0.5. Hz oriented perpendicularly to the direction of stretching after 10. min of exposure. On the other hand, the orientation of paxillin knockdown ECs was abolished at 10. min, but it was observed after 60. min of cyclic stretching exposure. Immunofluorescent microscopy revealed that accumulation and redistribution of FA proteins, including focal adhesion kinase (FAK) and integrin β1, were observed at 10. min of exposure to cyclic stretching in non-treated ECs. The accumulation of FAK and integrin β1 was not prominent in paxillin knockdown ECs under static conditions and after 10. min of exposure to cyclic stretching. However, we found that accumulation of FA proteins in paxillin knockdown ECs at 30 and 60. min was similar to that in non-transfected ECs. Because paxillin is an adaptor protein offering binding sites for FAK and integrin β1, which are critical molecules for the early signaling events of focal adhesion formation in ECs, these results suggest that paxillin is required for the early phase of EC orientation in response to cyclic stretching by scaffolding for accumulation of FA proteins.

本文言語英語
ページ(範囲)708-713
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
418
4
DOI
出版ステータス出版済み - 2 24 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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