Role of plasma as activator and cofactor in phosphorylation catalyzed by protein kinase C

Jeong Hun Kang, Daisuke Asai, Jun Oishi, Kenji Kawamura, Riki Toita, Yuhua Jiang, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The purpose of this study was to investigate whether plasma can influence the phosphorylation of protein kinase C (PKC). Lysate samples were prepared from normal skin or melanoma tissue and were reacted with a PKC peptide substrate in the presence or absence of plasma. In normal skin tissue lysates, the phosphorylation rates were much lower than those in melanoma tissue lysates. However, the level of phosphorylated peptide was increased in both normal skin and melanoma tissue lysates if plasma was present. Phosphorylation rates in the samples taken from the centre of B16 melanoma tissue were lower than those in samples taken from the edge. Moreover, addition of activator and/or cofactors (diacylglycerol, phosphatidylserine and/or Ca2+) of PKC, or plasma to the lysates contaminated by plasma had no effect on phosphorylation rates for the peptide substrate. These results indicate that plasma can play a role of activator and cofactor for substrate phosphorylation.

元の言語英語
ページ(範囲)70-75
ページ数6
ジャーナルCell Biochemistry and Function
26
発行部数1
DOI
出版物ステータス出版済み - 1 1 2008

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Phosphorylation
Protein Kinase C
Plasmas
Tissue
Melanoma
Skin
Substrates
Peptides
Experimental Melanomas
C-Peptide
Phosphatidylserines
Diglycerides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

これを引用

Role of plasma as activator and cofactor in phosphorylation catalyzed by protein kinase C. / Kang, Jeong Hun; Asai, Daisuke; Oishi, Jun; Kawamura, Kenji; Toita, Riki; Jiang, Yuhua; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki.

:: Cell Biochemistry and Function, 巻 26, 番号 1, 01.01.2008, p. 70-75.

研究成果: ジャーナルへの寄稿記事

Kang, Jeong Hun ; Asai, Daisuke ; Oishi, Jun ; Kawamura, Kenji ; Toita, Riki ; Jiang, Yuhua ; Mori, Takeshi ; Niidome, Takuro ; Katayama, Yoshiki. / Role of plasma as activator and cofactor in phosphorylation catalyzed by protein kinase C. :: Cell Biochemistry and Function. 2008 ; 巻 26, 番号 1. pp. 70-75.
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AU - Kang, Jeong Hun

AU - Asai, Daisuke

AU - Oishi, Jun

AU - Kawamura, Kenji

AU - Toita, Riki

AU - Jiang, Yuhua

AU - Mori, Takeshi

AU - Niidome, Takuro

AU - Katayama, Yoshiki

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AB - The purpose of this study was to investigate whether plasma can influence the phosphorylation of protein kinase C (PKC). Lysate samples were prepared from normal skin or melanoma tissue and were reacted with a PKC peptide substrate in the presence or absence of plasma. In normal skin tissue lysates, the phosphorylation rates were much lower than those in melanoma tissue lysates. However, the level of phosphorylated peptide was increased in both normal skin and melanoma tissue lysates if plasma was present. Phosphorylation rates in the samples taken from the centre of B16 melanoma tissue were lower than those in samples taken from the edge. Moreover, addition of activator and/or cofactors (diacylglycerol, phosphatidylserine and/or Ca2+) of PKC, or plasma to the lysates contaminated by plasma had no effect on phosphorylation rates for the peptide substrate. These results indicate that plasma can play a role of activator and cofactor for substrate phosphorylation.

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