Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans

Y. Murakami, Yoshihisa Yamashita, Y. Nakano, H. O. Ito, H. Yu, T. Koga

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

To make clear the role of the C terminus of a surface protein antigen (PAc) of Streptococcus mutans, stepwise truncations beginning at the C terminus of PAc were performed by utilizing site-directed mutagenesis. A remarkable increase in the amount of cell-free PAc was observed upon deletion of four or more amino acid residues at the C terminus. On the other hand, the amount of cell surface PAc gradually decreased when increasing numbers (four or more) of amino acid residues were deleted at the C terminus, and deletion of six amino acids involving both the total charged tail and Leu, an amino acid residue immediately upstream of the charged tail, resulted in a drastic reduction in the amount of cell surface PAc. These results indicate that the cytoplasmic charged tail and Leu residue are required for cell surface localization of PAc in S. mutans.

元の言語英語
ページ(範囲)1531-1535
ページ数5
ジャーナルInfection and Immunity
65
発行部数4
出版物ステータス出版済み - 4 15 1997

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Streptococcus mutans
Surface Antigens
Membrane Proteins
Amino Acids
Site-Directed Mutagenesis

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

これを引用

Murakami, Y., Yamashita, Y., Nakano, Y., Ito, H. O., Yu, H., & Koga, T. (1997). Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans. Infection and Immunity, 65(4), 1531-1535.

Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans. / Murakami, Y.; Yamashita, Yoshihisa; Nakano, Y.; Ito, H. O.; Yu, H.; Koga, T.

:: Infection and Immunity, 巻 65, 番号 4, 15.04.1997, p. 1531-1535.

研究成果: ジャーナルへの寄稿記事

Murakami, Y, Yamashita, Y, Nakano, Y, Ito, HO, Yu, H & Koga, T 1997, 'Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans', Infection and Immunity, 巻. 65, 番号 4, pp. 1531-1535.
Murakami, Y. ; Yamashita, Yoshihisa ; Nakano, Y. ; Ito, H. O. ; Yu, H. ; Koga, T. / Role of the charged tail in localization of a surface protein antigen of Streptococcus mutans. :: Infection and Immunity. 1997 ; 巻 65, 番号 4. pp. 1531-1535.
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abstract = "To make clear the role of the C terminus of a surface protein antigen (PAc) of Streptococcus mutans, stepwise truncations beginning at the C terminus of PAc were performed by utilizing site-directed mutagenesis. A remarkable increase in the amount of cell-free PAc was observed upon deletion of four or more amino acid residues at the C terminus. On the other hand, the amount of cell surface PAc gradually decreased when increasing numbers (four or more) of amino acid residues were deleted at the C terminus, and deletion of six amino acids involving both the total charged tail and Leu, an amino acid residue immediately upstream of the charged tail, resulted in a drastic reduction in the amount of cell surface PAc. These results indicate that the cytoplasmic charged tail and Leu residue are required for cell surface localization of PAc in S. mutans.",
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AU - Yamashita, Yoshihisa

AU - Nakano, Y.

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AU - Yu, H.

AU - Koga, T.

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AB - To make clear the role of the C terminus of a surface protein antigen (PAc) of Streptococcus mutans, stepwise truncations beginning at the C terminus of PAc were performed by utilizing site-directed mutagenesis. A remarkable increase in the amount of cell-free PAc was observed upon deletion of four or more amino acid residues at the C terminus. On the other hand, the amount of cell surface PAc gradually decreased when increasing numbers (four or more) of amino acid residues were deleted at the C terminus, and deletion of six amino acids involving both the total charged tail and Leu, an amino acid residue immediately upstream of the charged tail, resulted in a drastic reduction in the amount of cell surface PAc. These results indicate that the cytoplasmic charged tail and Leu residue are required for cell surface localization of PAc in S. mutans.

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