Roles for the two-hybrid system in exploration of the yeast protein interactome.

Takashi Ito, Kazuhisa Ota, Hiroyuki Kubota, Yoshihiro Yamaguchi, Tomoko Chiba, Kazumi Sakuraba, Mikio Yoshida

研究成果: ジャーナルへの寄稿評論記事

96 引用 (Scopus)

抄録

Comprehensive analysis of protein-protein interactions is a challenging endeavor of functional proteomics and has been best explored in the budding yeast. The yeast protein interactome analysis was achieved first by using the yeast two-hybrid system in a proteome-wide scale and next by large-scale mass spectrometric analysis of affinity-purified protein complexes. While these interaction data have led to a number of novel findings and the emergence of a single huge network containing thousands of proteins, they suffer many false signals and fall short of grasping the entire interactome. Thus, continuous efforts are necessary in both bioinformatics and experimentation to fully exploit these data and to proceed another step forward to the goal. Computational tools to integrate existing biological knowledge buried in literature and various functional genomic data with the interactome data are required for biological interpretation of the huge protein interaction network. Novel experimental methods have to be developed to detect weak, transient interactions involving low abundance proteins as well as to obtain clues to the biological role for each interaction. Since the yeast two-hybrid system can be used for the mapping of the interaction domains and the isolation of interaction-defective mutants, it would serve as a technical basis for the latter purpose, thereby playing another important role in the next phase of protein interactome research.

元の言語英語
ページ(範囲)561-566
ページ数6
ジャーナルMolecular & cellular proteomics : MCP
1
発行部数8
DOI
出版物ステータス出版済み - 8 2002
外部発表Yes

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Fungal Proteins
Hybrid systems
Two-Hybrid System Techniques
Proteins
Yeast
Protein Interaction Maps
Saccharomycetales
Proteome
Computational Biology
Proteomics
Bioinformatics
Research

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

これを引用

Roles for the two-hybrid system in exploration of the yeast protein interactome. / Ito, Takashi; Ota, Kazuhisa; Kubota, Hiroyuki; Yamaguchi, Yoshihiro; Chiba, Tomoko; Sakuraba, Kazumi; Yoshida, Mikio.

:: Molecular & cellular proteomics : MCP, 巻 1, 番号 8, 08.2002, p. 561-566.

研究成果: ジャーナルへの寄稿評論記事

Ito, Takashi ; Ota, Kazuhisa ; Kubota, Hiroyuki ; Yamaguchi, Yoshihiro ; Chiba, Tomoko ; Sakuraba, Kazumi ; Yoshida, Mikio. / Roles for the two-hybrid system in exploration of the yeast protein interactome. :: Molecular & cellular proteomics : MCP. 2002 ; 巻 1, 番号 8. pp. 561-566.
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AU - Yoshida, Mikio

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