Roles of silkworm endoplasmic reticulum chaperones in the secretion of recombinant proteins expressed by baculovirus system

Saki Imai, Takahiro Kusakabe, Jian Xu, Zhiqing Li, Shintaro Shirai, Hiroaki Mon, Daisuke Morokuma, Jae Man Lee

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

Baculovirus expression vector system (BEVS) is widely used for production of recombinant eukaryotic proteins in insect larvae or cultured cells. BEVS has advantages over bacterial expression system in producing post-translationally modified secreted proteins. However, for some unknown reason, it is very difficult for insects to secrete sufficiently for certain proteins of interest. To understand the reasons why insect cells fail to secrete some kinds of recombinant proteins, we here employed three mammalian proteins as targets, EPO, HGF, and Wnt3A, with different secretion levels in BEVS and investigated their mRNA transcriptions from the viral genome, subcellular localizations, and interactions with silkworm ER chaperones. Moreover, we observed that no significantly influence on the secretion amounts of all three proteins when depleting or overexpressing most endogenous ER chaperone genes in cultured silkworm cells. However, among all detected ER chaperones, the depletion of BiP severely decreased the recombinant protein secretion in BEVS, indicating the possible central role of Bip in silkworm secretion pathway.

本文言語英語
ページ(範囲)255-262
ページ数8
ジャーナルMolecular and cellular biochemistry
409
1-2
DOI
出版ステータス出版済み - 11月 1 2015

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 臨床生化学
  • 細胞生物学

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