Roles of the helicase and primase domain of the gene 4 protein of bacteriophage T7 in accessing the primase recognition site

Takahiro Kusakabe, Khandan Baradaran, Joonsoo Lee, Charles C. Richardson

研究成果: ジャーナルへの寄稿記事

30 引用 (Scopus)

抄録

The 63 kDa gene 4 protein of bacteriophage T7 provides both helicase and primase activities. The C-terminal helicase domain of the gene 4 protein is responsible for DNA-dependent NTP hydrolysis and for hexamer formation, whereas the N-terminal primase domain contains the zinc motif that is, in part, responsible for template-directed oligoribonucleotide synthesis. In the presence of β,γ-methylene dTTP, the protein forms a hexamer that surrounds and binds tightly to single-stranded DNA and consequently is unable to translocate to primase recognition sites, 5'-GTC-3', or to dissociate from the molecule to which it is bound. Nonetheless, in the presence of β,γ-methylene dTTP, it catalyzes the synthesis of pppAC dimers at primase sites on M13 DNA. When bound to single-stranded DNA in the presence of β,γ-methylene dTTP, the primase can function at recognition sites on the same molecule to which it is bound provided that a sufficient distance exists between the recognition site and the site to which it is bound. Furthermore, the primase bound to one DNA strand can function at a primase site located on a second DNA strand. The results indicate that the primase domain resides on the outside of the hexameric ring, a location that enables it to access sites distal to its site of binding.

元の言語英語
ページ(範囲)1542-1552
ページ数11
ジャーナルEMBO Journal
17
発行部数5
DOI
出版物ステータス出版済み - 3 2 1998
外部発表Yes

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DNA Primase
Single-Stranded DNA
DNA
Oligoribonucleotides
Molecules
Dimers
Zinc
Hydrolysis
Proteins
Genes
Binding Sites

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Roles of the helicase and primase domain of the gene 4 protein of bacteriophage T7 in accessing the primase recognition site. / Kusakabe, Takahiro; Baradaran, Khandan; Lee, Joonsoo; Richardson, Charles C.

:: EMBO Journal, 巻 17, 番号 5, 02.03.1998, p. 1542-1552.

研究成果: ジャーナルへの寄稿記事

Kusakabe, Takahiro ; Baradaran, Khandan ; Lee, Joonsoo ; Richardson, Charles C. / Roles of the helicase and primase domain of the gene 4 protein of bacteriophage T7 in accessing the primase recognition site. :: EMBO Journal. 1998 ; 巻 17, 番号 5. pp. 1542-1552.
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