Rotational diffusion analysis of polyethylene glycol induced protein interactions

Daisuke Takahashi, Shoji Yamashita, Om Prakash, Etsuko Nishimoto

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Protein intermolecular depletion interactions induced by polyethylene glycol (PEG) depend largely on its concentration and molecular weight. Herein, we investigated the effects of various concentrations and molecular weights of PEG on lysozyme interactions through the analysis of protein rotational diffusion, which is susceptible to intermolecular interactions at short range. To this end, we measured fluorescence anisotropy of fluorescein-tagged lysozyme added as a tracer in concentrated native lysozyme solutions and introduced a protein concentration-dependent interaction parameter, krot. The results show the nonmonotonic changes in krot as the concentrations of PEG10000 and 6000 are increased. The depletion attractions are characterized by the decrease in krot, indicating an increase of a degree at which protein rotational diffusion slows down. The influences of temperature on the lysozyme rotational diffusion and krot were also measured, and the validity of this approach was checked through comparison with the colloidal theory.

元の言語英語
ページ(範囲)11786-11792
ページ数7
ジャーナルJournal of Physical Chemistry B
115
発行部数41
DOI
出版物ステータス出版済み - 10 20 2011

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lysozyme
Muramidase
Polyethylene glycols
glycols
polyethylenes
Enzymes
proteins
Proteins
molecular weight
depletion
Molecular Weight
Molecular weight
interactions
Fluorescence Polarization
Fluorescein
attraction
tracers
Anisotropy
Fluorescence
fluorescence

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

これを引用

Rotational diffusion analysis of polyethylene glycol induced protein interactions. / Takahashi, Daisuke; Yamashita, Shoji; Prakash, Om; Nishimoto, Etsuko.

:: Journal of Physical Chemistry B, 巻 115, 番号 41, 20.10.2011, p. 11786-11792.

研究成果: ジャーナルへの寄稿記事

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