Rotational diffusion analysis of polyethylene glycol induced protein interactions

Daisuke Takahashi, Shoji Yamashita, Om Prakash, Etsuko Nishimoto

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

Protein intermolecular depletion interactions induced by polyethylene glycol (PEG) depend largely on its concentration and molecular weight. Herein, we investigated the effects of various concentrations and molecular weights of PEG on lysozyme interactions through the analysis of protein rotational diffusion, which is susceptible to intermolecular interactions at short range. To this end, we measured fluorescence anisotropy of fluorescein-tagged lysozyme added as a tracer in concentrated native lysozyme solutions and introduced a protein concentration-dependent interaction parameter, krot. The results show the nonmonotonic changes in krot as the concentrations of PEG10000 and 6000 are increased. The depletion attractions are characterized by the decrease in krot, indicating an increase of a degree at which protein rotational diffusion slows down. The influences of temperature on the lysozyme rotational diffusion and krot were also measured, and the validity of this approach was checked through comparison with the colloidal theory.

本文言語英語
ページ(範囲)11786-11792
ページ数7
ジャーナルJournal of Physical Chemistry B
115
41
DOI
出版ステータス出版済み - 10 20 2011

All Science Journal Classification (ASJC) codes

  • 物理化学および理論化学
  • 表面、皮膜および薄膜
  • 材料化学

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