S100A4 mRNA expression level is a predictor of radioresistance of pancreatic cancer cells

Shingo Kozono, Kenoki Ohuchida, Takao Ohtsuka, Lin Cui, Daiki Eguchi, Kenji Fujiwara, Ming Zhao, Kazuhiro Mizumoto, Masao Tanaka

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

Improving poor outcomes in patients with pancreatic cancer requires a greater understanding of the biological mechanisms contributing to radioresistance. We, therefore, sought to identify genes involved in the radioresistance of pancreatic cancer cells. Two pancreatic cancer cell lines, CFPAC-1 and Capan-1, were repeatedly exposed to radiation, establishing two radioresistant cell lines. Gene expression profiling using cDNA microarrays was performed to identify genes responsible for radioresistance. The levels of expression of mRNAs encoded by selected genes and their correlation with radiation dose resulting in 50% survival rate were analyzed in pancreatic cancer cell lines. The radiation dose resulting in a 50% survival rate was significantly higher in irradiated (IR) compared to parental CFPAC-1 cells (8.31±0.85 Gy vs. 2.14±0.04 Gy, P<0.0001), but was lower in IR compared with parental Capan-1 cells (2.66±0.24 Gy vs. 2.25±0.03Gy, P=0.04). cDNA microarray analysis identified 4 genes, including S100 calcium binding protein A4 (S100A4), overexpressed and 23 genes underexpressed in the IR compared with the parental cell lines. The levels of S100A4 mRNA expression were correlated with radiation dose resulting in a 50% survival rate (Pearson's test, R2=0.81, P=0.0025). S100A4 mRNA expression may predict radioresistance of pancreatic cancer cells and may play an important role in the poor response of pancreatic cancer cells to radiation therapy.

本文言語英語
ページ(範囲)1601-1608
ページ数8
ジャーナルOncology reports
30
4
DOI
出版ステータス出版済み - 10 2013

All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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