Sadaaki Iwanaga: Discovery of the lipopolysaccharide- and β-1,3-d-glucan-mediated proteolytic cascade and unique proteins in invertebrate immunity

Shun-Ichiro Kawabata, Tatsushi Muta

研究成果: ジャーナルへの寄稿評論記事

19 引用 (Scopus)

抄録

Horseshoe crab haemolymph contains a single type of cells, granular haemocytes, which are extremely sensitive to bacterial lipopolysaccharides (LPS) and lead to haemolymph coagulation. Sadaaki Iwanaga isolated protease zymogens from the haemocytes and reconstituted LPS and β-1,3-d-glucans-mediated haemolymph coagulation. This led to the first discovery of a proteolytic cascade triggered by pathogen-associated molecular patterns, an important milestone for studies on invertebrate innate immunity. Moreover, he separated components derived from haemocyte granules and haemolymph plasma, and consequently identified unique defense molecules, such as lectins, serpins, cystatins, antimicrobial substances and substrates for transglutaminase. Through steady and persistent studies on the horseshoe crab host defense system, he made great progress in the field. Now we know that LPS-induced haemocyte exocytosis leads not only to coagulation but also activates a sophisticated immune response network that coordinately induces pathogen recognition, elimination and wound healing.

元の言語英語
ページ(範囲)611-618
ページ数8
ジャーナルJournal of biochemistry
147
発行部数5
DOI
出版物ステータス出版済み - 1 1 2010

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Hemocytes
Glucans
Hemolymph
Invertebrates
Coagulation
Lipopolysaccharides
Immunity
Horseshoe Crabs
Cystatins
Serpins
Enzyme Precursors
Proteins
Transglutaminases
Pathogens
Lectins
Exocytosis
Peptide Hydrolases
Innate Immunity
Wound Healing
Plasmas

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

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abstract = "Horseshoe crab haemolymph contains a single type of cells, granular haemocytes, which are extremely sensitive to bacterial lipopolysaccharides (LPS) and lead to haemolymph coagulation. Sadaaki Iwanaga isolated protease zymogens from the haemocytes and reconstituted LPS and β-1,3-d-glucans-mediated haemolymph coagulation. This led to the first discovery of a proteolytic cascade triggered by pathogen-associated molecular patterns, an important milestone for studies on invertebrate innate immunity. Moreover, he separated components derived from haemocyte granules and haemolymph plasma, and consequently identified unique defense molecules, such as lectins, serpins, cystatins, antimicrobial substances and substrates for transglutaminase. Through steady and persistent studies on the horseshoe crab host defense system, he made great progress in the field. Now we know that LPS-induced haemocyte exocytosis leads not only to coagulation but also activates a sophisticated immune response network that coordinately induces pathogen recognition, elimination and wound healing.",
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