Salt-induced changes in the subunit structure of the Bacillus stearothermophilus lipoate acetyltransferase

Yuichi Shigeoka, Tetsuro Fujisawa, Satoshi Teshiba, Hisayoshi Fukumori, Kohji Yamamoto, Yutaka Banno, Yoichi Aso

研究成果: ジャーナルへの寄稿記事

抄録

The Bacillus stearothermophilus lipoate acetyltransferase (E2), composed of sixty identical, subunits is the core component of the pyruvate dehydrogenase complex (PDC). E2 polypeptide is composed of LD, PSBD, and CD domains. Most studies had focused on a truncated E2 that is deficient in LD and PSBD, because CD mainly contributes to maintaining the multimeric structure. We examined salt-induced changes in E2 without truncation and constructed reaction models. We speculate that in the presence of KCl, E2 is dissociated into a monomer and then assembled into an aggregative complex (CA) and a quasi-stable complex (CQ). CA was larger than CQ, but smaller than intact E2. CA and CQ were dominant complexes at about neutral pH and at basic pH respectively. PDC, in which PSBD is occupied by other components, and a truncated E2 undergo dissociation only. LD-PSBD region besides CD might then contribute to the partial association of dissociated E2.

元の言語英語
ページ(範囲)1637-1644
ページ数8
ジャーナルBioscience, Biotechnology and Biochemistry
77
発行部数8
DOI
出版物ステータス出版済み - 9 16 2013

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Dihydrolipoyllysine-Residue Acetyltransferase
Pyruvate Dehydrogenase Complex
Geobacillus stearothermophilus
Bacilli
Salts
Monomers
Association reactions
Peptides

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

これを引用

Salt-induced changes in the subunit structure of the Bacillus stearothermophilus lipoate acetyltransferase. / Shigeoka, Yuichi; Fujisawa, Tetsuro; Teshiba, Satoshi; Fukumori, Hisayoshi; Yamamoto, Kohji; Banno, Yutaka; Aso, Yoichi.

:: Bioscience, Biotechnology and Biochemistry, 巻 77, 番号 8, 16.09.2013, p. 1637-1644.

研究成果: ジャーナルへの寄稿記事

Shigeoka, Yuichi ; Fujisawa, Tetsuro ; Teshiba, Satoshi ; Fukumori, Hisayoshi ; Yamamoto, Kohji ; Banno, Yutaka ; Aso, Yoichi. / Salt-induced changes in the subunit structure of the Bacillus stearothermophilus lipoate acetyltransferase. :: Bioscience, Biotechnology and Biochemistry. 2013 ; 巻 77, 番号 8. pp. 1637-1644.
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AU - Yamamoto, Kohji

AU - Banno, Yutaka

AU - Aso, Yoichi

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