Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury

Yusuke Sato, Mai Khoi Q. Do, Takahiro Suzuki, Hideaki Ohtsubo, Wataru Mizunoya, Mako Nakamura, Mitsuhiro Furuse, Yoshihide Ikeuchi, Ryuichi Tatsumi

研究成果: ジャーナルへの寄稿記事

16 引用 (Scopus)

抄録

Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed a heretofore unexplored role of satellite cells as a key source of a secreted neural chemorepellent semaphorin 3A (Sema3A) expression. In order to verify this concept, there is still a critical need to provide direct evidence to show the up-regulation of Sema3A protein in satellite cells in vivo upon muscle injury. The present study employed a Sema3A/MyoD double-immunohistochemical staining for cryo-sections prepared from cardiotoxin injected gastrocnemius muscle of adult mouse lower hind-limb. Results clearly demonstrated that Sema3A expression was up-regulated in myogenic differentiation-positive satellite cells at 4-12 days post-injury period, the time that corresponds to the cell differentiation phase characterized by increasing myogenin messenger RNA expression. This direct proof encourages a possible implication of satellite cells in the spatiotemporal regulation of extracellular Sema3A concentrations, which potentially ensures coordinating a delay in neurite sprouting and re-attachment of motoneuron terminals onto damaged muscle fibers early in muscle regeneration in synchrony with recovery of muscle-fiber integrity.

元の言語英語
ページ(範囲)185-189
ページ数5
ジャーナルAnimal Science Journal
84
発行部数2
DOI
出版物ステータス出版済み - 2 1 2013

Fingerprint

Semaphorin-3A
Muscles
muscles
Wounds and Injuries
muscle fibers
cells
Cardiotoxins
neurites
muscle development
Myogenin
innervation
motor neurons
messenger RNA
sprouting
limbs (animal)
axons
cell differentiation
Motor Neurons
Neurites
Regeneration

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

これを引用

Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury. / Sato, Yusuke; Do, Mai Khoi Q.; Suzuki, Takahiro; Ohtsubo, Hideaki; Mizunoya, Wataru; Nakamura, Mako; Furuse, Mitsuhiro; Ikeuchi, Yoshihide; Tatsumi, Ryuichi.

:: Animal Science Journal, 巻 84, 番号 2, 01.02.2013, p. 185-189.

研究成果: ジャーナルへの寄稿記事

Sato, Yusuke ; Do, Mai Khoi Q. ; Suzuki, Takahiro ; Ohtsubo, Hideaki ; Mizunoya, Wataru ; Nakamura, Mako ; Furuse, Mitsuhiro ; Ikeuchi, Yoshihide ; Tatsumi, Ryuichi. / Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury. :: Animal Science Journal. 2013 ; 巻 84, 番号 2. pp. 185-189.
@article{0ec391d14cd3476781d12710cae2bf45,
title = "Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury",
abstract = "Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed a heretofore unexplored role of satellite cells as a key source of a secreted neural chemorepellent semaphorin 3A (Sema3A) expression. In order to verify this concept, there is still a critical need to provide direct evidence to show the up-regulation of Sema3A protein in satellite cells in vivo upon muscle injury. The present study employed a Sema3A/MyoD double-immunohistochemical staining for cryo-sections prepared from cardiotoxin injected gastrocnemius muscle of adult mouse lower hind-limb. Results clearly demonstrated that Sema3A expression was up-regulated in myogenic differentiation-positive satellite cells at 4-12 days post-injury period, the time that corresponds to the cell differentiation phase characterized by increasing myogenin messenger RNA expression. This direct proof encourages a possible implication of satellite cells in the spatiotemporal regulation of extracellular Sema3A concentrations, which potentially ensures coordinating a delay in neurite sprouting and re-attachment of motoneuron terminals onto damaged muscle fibers early in muscle regeneration in synchrony with recovery of muscle-fiber integrity.",
author = "Yusuke Sato and Do, {Mai Khoi Q.} and Takahiro Suzuki and Hideaki Ohtsubo and Wataru Mizunoya and Mako Nakamura and Mitsuhiro Furuse and Yoshihide Ikeuchi and Ryuichi Tatsumi",
year = "2013",
month = "2",
day = "1",
doi = "10.1111/asj.12014",
language = "English",
volume = "84",
pages = "185--189",
journal = "Animal Science Journal",
issn = "1344-3941",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury

AU - Sato, Yusuke

AU - Do, Mai Khoi Q.

AU - Suzuki, Takahiro

AU - Ohtsubo, Hideaki

AU - Mizunoya, Wataru

AU - Nakamura, Mako

AU - Furuse, Mitsuhiro

AU - Ikeuchi, Yoshihide

AU - Tatsumi, Ryuichi

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed a heretofore unexplored role of satellite cells as a key source of a secreted neural chemorepellent semaphorin 3A (Sema3A) expression. In order to verify this concept, there is still a critical need to provide direct evidence to show the up-regulation of Sema3A protein in satellite cells in vivo upon muscle injury. The present study employed a Sema3A/MyoD double-immunohistochemical staining for cryo-sections prepared from cardiotoxin injected gastrocnemius muscle of adult mouse lower hind-limb. Results clearly demonstrated that Sema3A expression was up-regulated in myogenic differentiation-positive satellite cells at 4-12 days post-injury period, the time that corresponds to the cell differentiation phase characterized by increasing myogenin messenger RNA expression. This direct proof encourages a possible implication of satellite cells in the spatiotemporal regulation of extracellular Sema3A concentrations, which potentially ensures coordinating a delay in neurite sprouting and re-attachment of motoneuron terminals onto damaged muscle fibers early in muscle regeneration in synchrony with recovery of muscle-fiber integrity.

AB - Regenerative mechanisms that regulate intramuscular motor innervation. including configuration of the neuromuscular connections are thought to reside in the spatiotemporal expression of axon-guidance molecules. Our previous studies proposed a heretofore unexplored role of satellite cells as a key source of a secreted neural chemorepellent semaphorin 3A (Sema3A) expression. In order to verify this concept, there is still a critical need to provide direct evidence to show the up-regulation of Sema3A protein in satellite cells in vivo upon muscle injury. The present study employed a Sema3A/MyoD double-immunohistochemical staining for cryo-sections prepared from cardiotoxin injected gastrocnemius muscle of adult mouse lower hind-limb. Results clearly demonstrated that Sema3A expression was up-regulated in myogenic differentiation-positive satellite cells at 4-12 days post-injury period, the time that corresponds to the cell differentiation phase characterized by increasing myogenin messenger RNA expression. This direct proof encourages a possible implication of satellite cells in the spatiotemporal regulation of extracellular Sema3A concentrations, which potentially ensures coordinating a delay in neurite sprouting and re-attachment of motoneuron terminals onto damaged muscle fibers early in muscle regeneration in synchrony with recovery of muscle-fiber integrity.

UR - http://www.scopus.com/inward/record.url?scp=84873421580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873421580&partnerID=8YFLogxK

U2 - 10.1111/asj.12014

DO - 10.1111/asj.12014

M3 - Article

C2 - 23384361

AN - SCOPUS:84873421580

VL - 84

SP - 185

EP - 189

JO - Animal Science Journal

JF - Animal Science Journal

SN - 1344-3941

IS - 2

ER -