Scanning the Corynebacterium glutamicum R genome for high-efficiency secretion signal sequences

Keiro Watanabe, Yoshiki Tsuchida, Naoko Okibe, Haruhiko Teramoto, Nobuaki Suzuki, Masayuki Inui, Hideaki Yukawa

研究成果: Contribution to journalArticle査読

50 被引用数 (Scopus)

抄録

Systematic screening of secretion proteins using an approach based on the completely sequenced genome of Corynebacterium glutamicum R revealed 405 candidate signal peptides, 108 of which were able to heterologously secrete an active-form α-amylase derived from Geobacillus stearothermophilus. These comprised 90 general secretory (Sec)-type, 10 twin-arginine translocator (Tat)-type and eight Sec-type with presumptive lipobox peptides. Only Secand Tat-type signals directed high-efficiency secretion. In two assays, 11 of these signals resulted in 50- to 150-fold increased amounts of secreted α-amylase compared with the well-known corynebacterial secretory protein PS2. While the presence of an AXA motif at the cleavage sites was readily apparent, it was the presence of a glutamine residue adjacent to the cleavage site that may affect secretion efficiency.

本文言語英語
ページ(範囲)741-750
ページ数10
ジャーナルMicrobiology
155
3
DOI
出版ステータス出版済み - 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 微生物学

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