SCGB2A1 is a novel prognostic marker for colorectal cancer associated with chemoresistance and radioresistance

Koji Munakata, Mamoru Uemura, Ichiro Takemasa, Miyuki Ozaki, Masamitsu Konno, Junichi Nishimura, Taishi Hata, Tsunekazu Mizushima, Naotsugu Haraguchi, Shingo Noura, Masakazu Ikenaga, S. H.U. Okamura, Mutsumi Fukunaga, Kohei Murata, Hirofumi Yamamoto, Yuichiro Doki, Masaki Mori

研究成果: Contribution to journalArticle査読

15 被引用数 (Scopus)

抄録

We recently showed that liver metastatic tissue from patients with colorectal cancer (CRC) was a useful model for identifying novel, hypoxia-inducible genes and prognostic markers. We showed that the expression of secretoglobin, family 2A, member 1 (SCGB2A1) was a potential prognostic factor for CRC. Here, we further evaluated the prognostic impact and function of SCGB2A1 in 222 patients with CRC. The impact of SCGB2A1 expression on disease-free survival (DFS) and overall survival (OS) was assessed with mRNA expression profiling. The function of SCGB2A1 was analyzed by evaluating mRNA expression profiles in cells derived from patients with CRC and by testing the effects of transfecting SCGB2A1 into different CRC-derived cell lines. We evaluated the effects of SCGB2A1 on proliferation, chemosensitivity, radiation sensitivity and sphere formation. Univariate and multivariate analyses indicated that the expression of SCGB2A1 was an independent prognostic factor for CRC (p<0.05), together with lymph node metastasis (p<0.05). Enforced expression of SCGB2A1 in CRC-derived cell lines promoted proliferation (DLD1, SW480 and LoVo cells; p<0.05), decreased chemosensitivity to 5-fluorouracil and oxaliplatin (DLD1 and SW480 cell lines; p<0.05), and significantly increased the viability of irradiated cells (DLD1, SW480 and LoVo cell lines; p<0.05). SCGB2A1 expression was also correlated to cancer stemness-related genes (Wnt, Zeb1 and Twist). Consistent with this correlation, SCGB2A1 expressing cells (SW480) showed increased sphere formation (p<0.05). These results indicated that SCGB2A1 represented a novel, prognostic factor for CRC, and that expression of SCGB2A1 correlated with chemoresistance, radioresistance and cancer cell stemness.

本文言語英語
ページ(範囲)1521-1528
ページ数8
ジャーナルInternational journal of oncology
44
5
DOI
出版ステータス出版済み - 5 2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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