Scheduled conversion of replication complex architecture at replication origins of Saccharomyces cerevisiae during the cell cycle

Ryusuke Tadokoro, Masako Fujita, Hitoshi Miura, Katsuhiko Shirahige, Hiroshi Yoshikawa, Toshiki Tsurimoto, Chikashi Obuse

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

Replication of DNA within Saccharomyces cerevisiae chromosomes is initiated from multiple origins, whose activation follow their own inherent time schedules during the S phase of the cell cycle. It has been demonstrated that a characteristic replicative complex (RC) that includes an origin recognition complex is formed at each origin and shifts between post- and pre-replicative states during the cell cycle. We wanted to determine whether there was an association between this shift in the state of the RC and firing events at replication origins. Time course analyses of RC architecture using LTV-footprinting with synchronously growing cells revealed that pre-replicative states at both early and late firing origins appeared simultaneously during late M phase, remained in this state during G1 phase, and converted to the post-replicative state at various times during S phase. Because the conversion of the origin footprinting profiles and origin firing, as assessed by two-dimensional gel electrophoresis, occurred concomitantly at each origin, then these two events must be closely related. However, conversion of the late firing origin occurred without actual firing. This was observed when the late origin was suppressed in clb5-deficient cells and a replication fork originating from an outside origin replicated the late origin passively. This mechanism ensures that replication at each chromosomal locus occurs only once per cell cycle by shifting existing pre-RCs to the post-RC state, when it is replicated without firing.

本文言語英語
ページ(範囲)15881-15889
ページ数9
ジャーナルJournal of Biological Chemistry
277
18
DOI
出版ステータス出版済み - 5 3 2002
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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