Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier

Teruaki Hasegawa, Tomohisa Fujisawa, Munenori Numata, Takahiro Matsumoto, Mariko Umeda, Ryouji Karinaga, Masami Mizu, Kazuya Koumoto, Taro Kimura, Shiro Okumura, Kazuo Sakurai, Seiji Shinkai

研究成果: Contribution to journalArticle査読

22 被引用数 (Scopus)

抄録

Schizophyllans carrying β-lactoside and α-mannoside appendages were prepared from native schizophyllan through NaIO4 oxidation followed by reductive amination using aminoethyl-β-lactoside and α-mannoside, respectively. The resulting schizophyllans form stable macromolecular complexes with polynucleotides, such as poly(C) and poly(dA). Specific affinity between these macromolecular complexes and saccharide-binding proteins was demonstrated by surface plasmon resonance and agarose gel staining assays. β-Lactoside-appended schizophyllan enhanced an antisense activity in hepatocytes which express lactoside-binding proteins on their cell surfaces.

本文言語英語
ページ(範囲)3091-3098
ページ数8
ジャーナルOrganic and Biomolecular Chemistry
2
21
DOI
出版ステータス出版済み - 11 7 2004

All Science Journal Classification (ASJC) codes

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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