Schizosaccharomyces pombe UDP-galactose transporter: Identification of its functional form through cDNA cloning and expression in mammalian cells

Hiroaki Segawa, Nobuhiro Ishida, Kaoru Takegawa, Masao Kawakita

研究成果: ジャーナルへの寄稿学術誌査読

21 被引用数 (Scopus)

抄録

The Schizosaccharomyces pombe UDP-galactose transporter cDNA (SpUGT cDNA), encoding the product of the gms1+ gene which consists of two exon sequences separated by a 173-bp intron, was cloned by RT-PCR. Its product, a hydrophobic protein of 353 amino acid residues resembling its human counterpart, was expressed in the Golgi membranes of UDP-galactose transporter-deficient Lec8 cells, and complemented the genetic defect of the mutant cells. This indicated that SpUGT cDNA encodes the functional S. pombe UDP-galactose transporter. The product of an ORF found in the second exon, which was previously assumed to be the S. pombe UDP-galactose transporter, thus represents an inactive, truncated form of the SpUGT protein. Copyright (C) 1999 Federation of European Biochemical Societies.

本文言語英語
ページ(範囲)295-298
ページ数4
ジャーナルFEBS Letters
451
3
DOI
出版ステータス出版済み - 5月 28 1999
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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