Screening for imprinted genes by allelic message display: Identification of a paternally expressed gene Impact on mouse chromosome 18

Yuriko Hagiwara, Momoki Hirai, Kazutoshi Nishiyama, Ichiro Kanazawa, Takayuki Ueda, Yoshiyuki Sakaki, Takashi Ito

研究成果: Contribution to journalArticle査読

80 被引用数 (Scopus)

抄録

A systematic screen termed the allelic message display (AMD) was developed for the hunting of imprinted genes. In AMD, differential display PCR is adopted to image allelic expression status of multiple polymorphic transcripts in two parental mouse strains, reciprocal F1 hybrids and pooled backcross progenies. From the displayed patterns, paternally and maternally expressed transcripts can be unequivocally identified. The effectiveness of AMD screening was clearly demonstrated by the identification of a paternally expressed gene impact on mouse chromosome 18, the predicted product of which belongs to the YCR59c/yigZ hypothetical protein family composed of yeast and bacterial proteins with currently unknown function. In contrast with previous screening methods necessitating positional cloning efforts or generation of parthenogenetic embryos, this approach requires nothing particular but appropriately crossed mice and can be readily applied to any tissues at various developmental stages. Hence, AMD would considerably accelerate the identification of imprinted genes playing pivotal roles in mammalian development and the pathogenesis of various diseases.

本文言語英語
ページ(範囲)9249-9254
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
94
17
DOI
出版ステータス出版済み - 8 19 1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • 一般

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