Screening of soy protein-derived hypotriglyceridemic di-peptides in vitro and in vivo

Nao Inoue, Koji Nagao, Kotaro Sakata, Naomi Yamano, Pathma Elgoda Ranawakage Gunawardena, Seo Young Han, Toshiro Matsui, Toshihiro Nakamori, Hitoshi Furuta, Kiyoharu Takamatsu, Teruyoshi Yanagita

研究成果: Contribution to journalArticle

40 引用 (Scopus)

抜粋

Background: Soy protein and soy peptides have attracted considerable attention because of their potentially beneficial biological properties, including antihypertensive, anticarcinogenic, and hypolipidemic effects. Although soy protein isolate contains several bioactive peptides that have distinct physiological activities in lipid metabolism, it is not clear which peptide sequences are responsible for the triglyceride (TG)-lowering effects. In the present study, we investigated the effects of soy protein-derived peptides on lipid metabolism, especially TG metabolism, in HepG2 cells and obese Otsuka Long-Evans Tokushima fatty (OLETF) rats. Results: In the first experiment, we found that soy crude peptide (SCP)-LD3, which was prepared by hydrolyze of soy protein isolate with endo-type protease, showed hypolipidemic effects in HepG2 cells and OLETF rats. In the second experiment, we found that hydrophilic fraction, separated from SCP-LD3 with hydrophobic synthetic absorbent, revealed lipid-lowering effects in HepG2 cells and OLETF rats. In the third experiment, we found that Fraction-C (Frc-C) peptides, fractionated from hydrophilic peptides by gel permeation chromatography-high performance liquid chromatography, significantly reduced TG synthesis and apolipoprotein B (apoB) secretion in HepG2 cells. In the fourth experiment, we found that the fraction with 0.1% trifluoroacetic acid, isolated from Frc-C peptides by octadecylsilyl column chromatography, showed hypolipidemic effects in HepG2 cells. In the final experiment, we found that 3 di-peptides, Lys-Ala, Val-Lys, and Ser-Tyr, reduced TG synthesis, and Ser-Tyr additionally reduced apoB secretion in HepG2 cells. Conclusion: Novel active peptides with TG-lowering effects from soy protein have been isolated.

元の言語英語
記事番号85
ジャーナルLipids in Health and Disease
10
DOI
出版物ステータス出版済み - 2011

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

フィンガープリント Screening of soy protein-derived hypotriglyceridemic di-peptides in vitro and in vivo' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Inoue, N., Nagao, K., Sakata, K., Yamano, N., Gunawardena, P. E. R., Han, S. Y., Matsui, T., Nakamori, T., Furuta, H., Takamatsu, K., & Yanagita, T. (2011). Screening of soy protein-derived hypotriglyceridemic di-peptides in vitro and in vivo. Lipids in Health and Disease, 10, [85]. https://doi.org/10.1186/1476-511X-10-85