Secretory cell immunoscreening assay - A highly sensitive screening method for secretory cells

Kiichiro Teruya, Sanetaka Shirahata, Takahiro Yano, Kiyohiko Seki, Hirofumi Tachibana, Hideya Ohashi, Hiroki Murakami

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

We have developed a rapid and sensitive screening method to detect the secretory product from transformed secretory cells. Polyclonal antibodies against the target secretory protein are first adsorbed onto a nitrocellulose membrane and are then called solid-phase antibodies. Then this membrane is placed on top of target protein-secreting adherent animal cell colonies. After trapping the secreted protein on the membrane, the protein is detected using the highly sensitive enhanced chemiluminescence detection system. Thus, this method is named the secretory cell immunoscreening assay (SCISA). We have screened recombinant BHK-21 cells, which secrete human interleukin-6 (hIL-6) for highly productive clones. With the SCISA method, it is possible to detect less than 100 pg of hIL-6 secreted by a single colony. We are also able to select highly productive colonies rapidly and easily from a large sample population of transformed cell colonies.

元の言語英語
ページ(範囲)468-473
ページ数6
ジャーナルAnalytical Biochemistry
214
発行部数2
DOI
出版物ステータス出版済み - 11 1 1993

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Assays
Screening
Interleukin-6
Membranes
Proteins
Collodion
Chemiluminescence
Antibodies
Membrane Proteins
Animals
Cells
Luminescence
Clone Cells
Population

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Secretory cell immunoscreening assay - A highly sensitive screening method for secretory cells. / Teruya, Kiichiro; Shirahata, Sanetaka; Yano, Takahiro; Seki, Kiyohiko; Tachibana, Hirofumi; Ohashi, Hideya; Murakami, Hiroki.

:: Analytical Biochemistry, 巻 214, 番号 2, 01.11.1993, p. 468-473.

研究成果: ジャーナルへの寄稿記事

Teruya, Kiichiro ; Shirahata, Sanetaka ; Yano, Takahiro ; Seki, Kiyohiko ; Tachibana, Hirofumi ; Ohashi, Hideya ; Murakami, Hiroki. / Secretory cell immunoscreening assay - A highly sensitive screening method for secretory cells. :: Analytical Biochemistry. 1993 ; 巻 214, 番号 2. pp. 468-473.
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AU - Tachibana, Hirofumi

AU - Ohashi, Hideya

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