Selection of discriminant markers for authentication of asian palm civet coffee (Kopi Luwak): A metabolomics approach

Udi Jumhawan, Sastia Prama Putri, Yusianto, Erly Marwani, Takeshi Bamba, Eiichiro Fukusaki

研究成果: ジャーナルへの寄稿記事

46 引用 (Scopus)

抄録

Kopi Luwak, an exotic Indonesian coffee, is made from coffee berries that have been eaten by the Asian palm civet (Paradoxurus hermaphroditus). Despite being known as the world's most expensive coffee, there is no reliable, standardized method for determining its authenticity. GC-MS-based multimarker profiling was employed to explore significant metabolites as discriminant markers for authentication. Extracts of 21 coffee beans (Coffea arabica and Coffea canephora) from three cultivation areas were analyzed and subjected to multivariate analyses, principal component analysis, and orthogonal projection to latent structures discriminant analysis. Citric acid, malic acid, and the inositol/pyroglutamic acid ratio were selected for further verification by evaluating their differentiating abilities against various commercial coffee products. The markers demonstrated potential application in the differentiation of original, fake Kopi Luwak, regular coffee, and coffee blend samples with 50 wt % Kopi Luwak content. This is the first report to address the selection and successful validation of discriminant markers for the authentication of Kopi Luwak.

元の言語英語
ページ(範囲)7994-8001
ページ数8
ジャーナルJournal of Agricultural and Food Chemistry
61
発行部数33
DOI
出版物ステータス出版済み - 8 21 2013
外部発表Yes

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Viverridae
Coffee
Metabolomics
metabolomics
Authentication
coffee products
Coffea canephora
coffee beans
Coffea arabica
malic acid
citric acid
discriminant analysis
small fruits
Coffea
principal component analysis
metabolites
acids
extracts
Pyrrolidonecarboxylic Acid
sampling

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

これを引用

Selection of discriminant markers for authentication of asian palm civet coffee (Kopi Luwak) : A metabolomics approach. / Jumhawan, Udi; Putri, Sastia Prama; Yusianto; Marwani, Erly; Bamba, Takeshi; Fukusaki, Eiichiro.

:: Journal of Agricultural and Food Chemistry, 巻 61, 番号 33, 21.08.2013, p. 7994-8001.

研究成果: ジャーナルへの寄稿記事

Jumhawan, Udi ; Putri, Sastia Prama ; Yusianto ; Marwani, Erly ; Bamba, Takeshi ; Fukusaki, Eiichiro. / Selection of discriminant markers for authentication of asian palm civet coffee (Kopi Luwak) : A metabolomics approach. :: Journal of Agricultural and Food Chemistry. 2013 ; 巻 61, 番号 33. pp. 7994-8001.
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abstract = "Kopi Luwak, an exotic Indonesian coffee, is made from coffee berries that have been eaten by the Asian palm civet (Paradoxurus hermaphroditus). Despite being known as the world's most expensive coffee, there is no reliable, standardized method for determining its authenticity. GC-MS-based multimarker profiling was employed to explore significant metabolites as discriminant markers for authentication. Extracts of 21 coffee beans (Coffea arabica and Coffea canephora) from three cultivation areas were analyzed and subjected to multivariate analyses, principal component analysis, and orthogonal projection to latent structures discriminant analysis. Citric acid, malic acid, and the inositol/pyroglutamic acid ratio were selected for further verification by evaluating their differentiating abilities against various commercial coffee products. The markers demonstrated potential application in the differentiation of original, fake Kopi Luwak, regular coffee, and coffee blend samples with 50 wt {\%} Kopi Luwak content. This is the first report to address the selection and successful validation of discriminant markers for the authentication of Kopi Luwak.",
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