Selective adsorption of EDA(+)fibronectin and antithronbin III by sufonated polysaccharide

We have reported that selective adsorption of EDA(+)fibronectin (EDA(+)FN) and plasma FN in cryogel using heparin-immobilized absorbents. However, the absorbent could not display the superior selectivity for antithronbin III(ATIII). In this work, we tried to prepare the materials which remains specific affinity for EDAFN except for the adsorbability to ATIII. Gellan was sulfonated chemically and immobilized on surfaces of substrate. Then, their properties were evaluated in vitro. As the results, we have succeeded to prepare material just fit to our purpose. 90% of EDAFN, 20% of total fibronectin and 8% of ATIII in plasma was adsorbed with the gellan sulfate (25% of hydroxy group was substitute).