Selective detection of NADPH among four pyridine-nucleotide cofactors by a fluorescent probe based on aggregation-induced emission

Takao Noguchi, Arnab Dawn, Daisuke Yoshihara, Youichi Tsuchiya, Tatsuhiro Yamamoto, Seiji Shinkai

研究成果: Contribution to journalArticle査読

24 被引用数 (Scopus)

抄録

A fluorescent sensor based on guanidinium-tethered tetraphenylethene (TPE) has been investigated toward the differentiation of pyridine nucleotide cofactors (NAD+, NADH, NADP+, and NADPH). TPE selectively recognizes NADPH possessing the higher tetra-anionic net-charge, resulting in the steep "turn-on" fluorescence increase. The comparative aggregation behaviors and fluorescence response studies of TPE on the four cofactors reveal that the critical aggregate concentration of TPE against NADPH correlates directly with the concentration threshold for the fluorescence response. These results establish that TPE can selectively differentiate NADPH over the other three cofactors by the steep aggregation-induced fluorescence response accompanied by the high signal-to-background contrast.

本文言語英語
ページ(範囲)779-784
ページ数6
ジャーナルMacromolecular rapid communications
34
9
DOI
出版ステータス出版済み - 5 14 2013

All Science Journal Classification (ASJC) codes

  • 有機化学
  • ポリマーおよびプラスチック
  • 材料化学

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