Selective extraction and recovery of cytochrome c by liquid-liquid extraction using a calix[6]arene carboxylic acid derivative

Tatsuya Oshima, Hiroaki Higuchi, Keisuke Ohto, Katsutoshi Inoue, Masahiro Goto

    研究成果: ジャーナルへの寄稿学術誌査読

    43 被引用数 (Scopus)

    抄録

    Recently, we reported that a calix[6]arene carboxylic acid derivative can selectively extract the lysinerich protein cytochrome c by interacting with amino groups on the protein surface. In the present article, quantitative extraction and recovery of cytochrome c using this calix[6]arene carboxylic acid derivative are described. Both adjustment of the pH under acidic conditions and addition of an alcohol are necessary to strip the extracted protein from an organic solution to an aqueous solution. Separation of cytochrome c and lysozyme using the calix[6] arene was achieved under the optimal conditions. In the forward extraction stage, 93% of the cytochrome c was extracted, while lysozyme remained in the solution. In the subsequent stripping stage, the extracted cytochrome c was quantitatively recovered in an aqueous solution. Finally, separation of these proteins, which have similar molecular weights and isoelectric points, was accomplished.

    本文言語英語
    ページ(範囲)7280-7284
    ページ数5
    ジャーナルLangmuir
    21
    16
    DOI
    出版ステータス出版済み - 8月 2 2005

    !!!All Science Journal Classification (ASJC) codes

    • 材料科学(全般)
    • 凝縮系物理学
    • 表面および界面
    • 分光学
    • 電気化学

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