Selective fluorescence detection of histamine based on ligand exchange mechanism and its application to biomonitoring

Daisuke Seto, Nobuaki Soh, Koji Nakano, Toshihiko Imato

研究成果: Contribution to journalArticle査読

24 被引用数 (Scopus)

抄録

We report on a novel histamine monitoring method by using a fluorescent probe, a complex between Ni2+ and calcein, based on a ligand exchange mechanism. The fluorescence intensity of this probe, which has been reduced due to effective quenching by Ni2+ ion, increases drastically by an addition of histamine. Furthermore, the probe shows high selectivity toward histamine among the various neurotransmitters in 0.1M phosphate buffer solution (pH 7.4). Biomonitoring studies to detect histamine released from RAW264 cells are successfully represented.

本文言語英語
ページ(範囲)135-139
ページ数5
ジャーナルAnalytical Biochemistry
404
2
DOI
出版ステータス出版済み - 9 2010

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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