Selective nitrosyl group transfer reaction to cytidine using oligonucleotides bearing S-nitrosothioguanosine.

Fumi Nagatsugi, Shizuka Nakayama, Monsur Md Ali, Shigeki Sasaki

研究成果: ジャーナルへの寄稿記事


Nitric oxide (NO) is an important endogenous regulatory molecule, and conversely, it is also known to be mutagenic because of its ability of nitrosylation and following deamination of nucleobases. In this study, we aimed at developing a useful tool for nitrosylation to a specific site of DNA by the use of ODN 2 incorporating S-nitroso thioguanosine. It has been demonstrated that the S-NO containing ODN 2 exhibits rapid and specific NO-transfer reaction to its complementary ODN 5 having dC or d(m)C at the target site. Thus, we have established an innovative method for the highly efficient and selective transfer of NO to cytidine and 5-methylcytidine.

ジャーナルNucleic acids symposium series (2004)
出版物ステータス出版済み - 1 1 2004


All Science Journal Classification (ASJC) codes

  • Medicine(all)