Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant

Yongil Yang, Hong Li Chou, Andrew J. Crofts, Laining Zhang, Li Tian, Haruhiko Washida, Masako Fukuda, Toshihiro Kumamaru, Oliver J. Oviedo, Shawn R. Starkenburg, Thomas W. Okita

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The transport of rice glutelin storage proteins to the storage vacuoles requires the Rab5 GTPase and its related guanine nucleotide exchange factor (Rab5-GEF). Loss of function of these membrane vesicular trafficking factors results in the initial secretion of storage proteins and later their partial engulfment by the plasma membrane to form an extracellular paramural body (PMB), an aborted endosome complex. Here, we show that in the rice Rab5-GEF mutant glup6, glutelin RNAs are specifically mislocalized from their normal location on the cisternal endoplasmic reticulum (ER) to the protein body-ER, and are also apparently translocated to the PMBs. We substantiated the association of mRNAs with this aborted endosome complex by RNA-seq of PMBs purified by flow cytometry. Two PMB-Associated groups of RNA were readily resolved: Those that were specifically enriched in this aborted complex and those that were highly expressed in the cytoplasm. Examination of the PMB-enriched RNAs indicated that they were not a random sampling of the glup6 transcriptome but, instead, encompassed only a few functional mRNA classes. Although specific autophagy is also an alternative mechanism, our results support the view that RNA localization may co-opt membrane vesicular trafficking, and that many RNAs that share function or intracellular location are co-Transported in developing rice seeds.

元の言語英語
ページ(範囲)5045-5058
ページ数14
ジャーナルJournal of Experimental Botany
69
発行部数21
DOI
出版物ステータス出版済み - 10 12 2018

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RNA
rice
mutants
Messenger RNA
glutelins
endosomes
Glutens
Endosomes
storage proteins
Endoplasmic Reticulum
endoplasmic reticulum
Guanine Nucleotide Exchange Factors
Proteins
protein bodies
Membranes
autophagy
GTP Phosphohydrolases
Autophagy
guanosinetriphosphatase
Vacuoles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

これを引用

Yang, Y., Chou, H. L., Crofts, A. J., Zhang, L., Tian, L., Washida, H., ... Okita, T. W. (2018). Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant. Journal of Experimental Botany, 69(21), 5045-5058. https://doi.org/10.1093/jxb/ery297

Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant. / Yang, Yongil; Chou, Hong Li; Crofts, Andrew J.; Zhang, Laining; Tian, Li; Washida, Haruhiko; Fukuda, Masako; Kumamaru, Toshihiro; Oviedo, Oliver J.; Starkenburg, Shawn R.; Okita, Thomas W.

:: Journal of Experimental Botany, 巻 69, 番号 21, 12.10.2018, p. 5045-5058.

研究成果: ジャーナルへの寄稿記事

Yang, Y, Chou, HL, Crofts, AJ, Zhang, L, Tian, L, Washida, H, Fukuda, M, Kumamaru, T, Oviedo, OJ, Starkenburg, SR & Okita, TW 2018, 'Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant', Journal of Experimental Botany, 巻. 69, 番号 21, pp. 5045-5058. https://doi.org/10.1093/jxb/ery297
Yang, Yongil ; Chou, Hong Li ; Crofts, Andrew J. ; Zhang, Laining ; Tian, Li ; Washida, Haruhiko ; Fukuda, Masako ; Kumamaru, Toshihiro ; Oviedo, Oliver J. ; Starkenburg, Shawn R. ; Okita, Thomas W. / Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant. :: Journal of Experimental Botany. 2018 ; 巻 69, 番号 21. pp. 5045-5058.
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abstract = "The transport of rice glutelin storage proteins to the storage vacuoles requires the Rab5 GTPase and its related guanine nucleotide exchange factor (Rab5-GEF). Loss of function of these membrane vesicular trafficking factors results in the initial secretion of storage proteins and later their partial engulfment by the plasma membrane to form an extracellular paramural body (PMB), an aborted endosome complex. Here, we show that in the rice Rab5-GEF mutant glup6, glutelin RNAs are specifically mislocalized from their normal location on the cisternal endoplasmic reticulum (ER) to the protein body-ER, and are also apparently translocated to the PMBs. We substantiated the association of mRNAs with this aborted endosome complex by RNA-seq of PMBs purified by flow cytometry. Two PMB-Associated groups of RNA were readily resolved: Those that were specifically enriched in this aborted complex and those that were highly expressed in the cytoplasm. Examination of the PMB-enriched RNAs indicated that they were not a random sampling of the glup6 transcriptome but, instead, encompassed only a few functional mRNA classes. Although specific autophagy is also an alternative mechanism, our results support the view that RNA localization may co-opt membrane vesicular trafficking, and that many RNAs that share function or intracellular location are co-Transported in developing rice seeds.",
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