Self-assembled amphotericin B is probably surrounded by ergosterol: Bimolecular interactions as evidenced by solid-state NMR and CD spectra

Yusuke Kasai, Nobuaki Matsumori, Yuichi Umegawa, Shigeru Matsuoka, Hiroyuki Ueno, Hiroki Ikeuchi, Tohru Oishi, Michio Murata

研究成果: ジャーナルへの寄稿記事

36 引用 (Scopus)

抄録

Amphotericin B (AmB) is thought to exert its pharmacological effects by forming a barrel-stave assembly with ergosterol in fungal membranes. To examine the interaction between AmB and ergosterol (Erg) or cholesterol (Cho), 13C- and 19F-labelled covalent conjugales were prepared as reported previously (N. Matsumori et al. Chem. Biol. 2004, 11, 673-679). The CD spectra of the conjugates in a membrane-bound form suggested that the distance between the heptaene moieties of the ergosterol conjugates AmB-C 2-(6-F)Erg 2 and AmB-C2Erg 3 is similar to that of AmB in ergosterol-containing membranes, but significantly larger than that of AmB in nonsterol or cholesterol-containing mem branes. These observations suggest that, as is the case with ergosterol-containing membranes, the conjugated sterol moiety prevents the close contact between the heptaene moieties within the membrane that would reduce channel conductivity of the AmB assemblies. To further investigate this bimolecular interaction, we recorded the solid-state NMR spectra of conjugates 2 and AmB-C2-(6-F)Cho 4. which are composed of uniformly 13C-labelled AmB and 6-fluorinuted ergosterol or cholesterol; the conjugates were expected to facilitate the estimation of distances between the fluorine and carbon atoms. By using rotor-synchronous double resonance (rotational echo double resonance of X cluster; RDX) experiments, we deduced the distance between the fluorine atom and its nearest carbon atom in the heptaene moiety of 2 to be less than 8.6 Å. This indicates that the B ring of ergosterol comes close to the AmB polyene moiety. A conformational search of the AmB-ergosterol conjugate by using distance constraints derived from the RDX results suggested that ergosterol molecules possibly surround the AmB assembly, which is in contrast with the conventional image in which ergosterol is inserted into AmB molecules.

元の言語英語
ページ(範囲)1178-1185
ページ数8
ジャーナルChemistry - A European Journal
14
発行部数4
DOI
出版物ステータス出版済み - 2 11 2008

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Ergosterol
Amphotericin B
Cholesterol
Nuclear magnetic resonance
Membranes
Fluorine
Atoms
Molecules
Carbon
Rotors
Polyenes
Sterols
Experiments

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Organic Chemistry

これを引用

Self-assembled amphotericin B is probably surrounded by ergosterol : Bimolecular interactions as evidenced by solid-state NMR and CD spectra. / Kasai, Yusuke; Matsumori, Nobuaki; Umegawa, Yuichi; Matsuoka, Shigeru; Ueno, Hiroyuki; Ikeuchi, Hiroki; Oishi, Tohru; Murata, Michio.

:: Chemistry - A European Journal, 巻 14, 番号 4, 11.02.2008, p. 1178-1185.

研究成果: ジャーナルへの寄稿記事

Kasai, Yusuke ; Matsumori, Nobuaki ; Umegawa, Yuichi ; Matsuoka, Shigeru ; Ueno, Hiroyuki ; Ikeuchi, Hiroki ; Oishi, Tohru ; Murata, Michio. / Self-assembled amphotericin B is probably surrounded by ergosterol : Bimolecular interactions as evidenced by solid-state NMR and CD spectra. :: Chemistry - A European Journal. 2008 ; 巻 14, 番号 4. pp. 1178-1185.
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abstract = "Amphotericin B (AmB) is thought to exert its pharmacological effects by forming a barrel-stave assembly with ergosterol in fungal membranes. To examine the interaction between AmB and ergosterol (Erg) or cholesterol (Cho), 13C- and 19F-labelled covalent conjugales were prepared as reported previously (N. Matsumori et al. Chem. Biol. 2004, 11, 673-679). The CD spectra of the conjugates in a membrane-bound form suggested that the distance between the heptaene moieties of the ergosterol conjugates AmB-C 2-(6-F)Erg 2 and AmB-C2Erg 3 is similar to that of AmB in ergosterol-containing membranes, but significantly larger than that of AmB in nonsterol or cholesterol-containing mem branes. These observations suggest that, as is the case with ergosterol-containing membranes, the conjugated sterol moiety prevents the close contact between the heptaene moieties within the membrane that would reduce channel conductivity of the AmB assemblies. To further investigate this bimolecular interaction, we recorded the solid-state NMR spectra of conjugates 2 and AmB-C2-(6-F)Cho 4. which are composed of uniformly 13C-labelled AmB and 6-fluorinuted ergosterol or cholesterol; the conjugates were expected to facilitate the estimation of distances between the fluorine and carbon atoms. By using rotor-synchronous double resonance (rotational echo double resonance of X cluster; RDX) experiments, we deduced the distance between the fluorine atom and its nearest carbon atom in the heptaene moiety of 2 to be less than 8.6 {\AA}. This indicates that the B ring of ergosterol comes close to the AmB polyene moiety. A conformational search of the AmB-ergosterol conjugate by using distance constraints derived from the RDX results suggested that ergosterol molecules possibly surround the AmB assembly, which is in contrast with the conventional image in which ergosterol is inserted into AmB molecules.",
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T2 - Bimolecular interactions as evidenced by solid-state NMR and CD spectra

AU - Kasai, Yusuke

AU - Matsumori, Nobuaki

AU - Umegawa, Yuichi

AU - Matsuoka, Shigeru

AU - Ueno, Hiroyuki

AU - Ikeuchi, Hiroki

AU - Oishi, Tohru

AU - Murata, Michio

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