Self-assembly immobilization of hyaluronan thiosemicarbazone on a gold surface for cell culture applications

Nobuo Tanaka, Yuka Yoshiike, Chiharu Yoshiyama, Takuya Kitaoka

研究成果: Contribution to journalArticle査読

12 被引用数 (Scopus)

抄録

Hyaluronan (HA)-fixed layers were successfully prepared on a gold coated glass plate via self-assembly chemisorption of HA derivatives whose aldehydic reducing ends were selectively modified with thiosemicarbazide (TSC). Quartz crystal microbalance analysis and X-ray photoelectron spectroscopy suggested formation of the HA layers on the gold surfaces through spontaneous S-Au interaction, resulting in stability in aqueous culture medium. Biological functions of the HA layers were investigated by cell adhesion assay using mouse fibroblast cells (NIH-3T3). Cell attachment to the hydrophilic HA surface (water contact angle ≈ 11°) was far superior to cell attachment to a sugar-free, hydrophobic gold substrate (≈57°), while a spin-coated film composed of water-soluble, TSC-free HA molecules made little contribution to cell adhesion. After 48 h incubation, the NIH-3T3 cells proliferated well only on the HA layers and retained their original viability. This novel approach to surface glyco-modification with biocompatible HA is expected to have potential applications as a biofunctional scaffold for cell culture applications.

本文言語英語
ページ(範囲)100-105
ページ数6
ジャーナルCarbohydrate Polymers
82
1
DOI
出版ステータス出版済み - 8 2 2010

All Science Journal Classification (ASJC) codes

  • 有機化学
  • ポリマーおよびプラスチック
  • 材料化学

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