抄録
Hyaluronan (HA)-fixed layers were successfully prepared on a gold coated glass plate via self-assembly chemisorption of HA derivatives whose aldehydic reducing ends were selectively modified with thiosemicarbazide (TSC). Quartz crystal microbalance analysis and X-ray photoelectron spectroscopy suggested formation of the HA layers on the gold surfaces through spontaneous S-Au interaction, resulting in stability in aqueous culture medium. Biological functions of the HA layers were investigated by cell adhesion assay using mouse fibroblast cells (NIH-3T3). Cell attachment to the hydrophilic HA surface (water contact angle ≈ 11°) was far superior to cell attachment to a sugar-free, hydrophobic gold substrate (≈57°), while a spin-coated film composed of water-soluble, TSC-free HA molecules made little contribution to cell adhesion. After 48 h incubation, the NIH-3T3 cells proliferated well only on the HA layers and retained their original viability. This novel approach to surface glyco-modification with biocompatible HA is expected to have potential applications as a biofunctional scaffold for cell culture applications.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 100-105 |
| ページ数 | 6 |
| ジャーナル | Carbohydrate Polymers |
| 巻 | 82 |
| 発行部数 | 1 |
| DOI | |
| 出版物ステータス | 出版済み - 8 2 2010 |
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All Science Journal Classification (ASJC) codes
- Organic Chemistry
- Polymers and Plastics
- Materials Chemistry
これを引用
Self-assembly immobilization of hyaluronan thiosemicarbazone on a gold surface for cell culture applications. / Tanaka, Nobuo; Yoshiike, Yuka; Yoshiyama, Chiharu; Kitaoka, Takuya.
：: Carbohydrate Polymers, 巻 82, 番号 1, 02.08.2010, p. 100-105.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Self-assembly immobilization of hyaluronan thiosemicarbazone on a gold surface for cell culture applications
AU - Tanaka, Nobuo
AU - Yoshiike, Yuka
AU - Yoshiyama, Chiharu
AU - Kitaoka, Takuya
PY - 2010/8/2
Y1 - 2010/8/2
N2 - Hyaluronan (HA)-fixed layers were successfully prepared on a gold coated glass plate via self-assembly chemisorption of HA derivatives whose aldehydic reducing ends were selectively modified with thiosemicarbazide (TSC). Quartz crystal microbalance analysis and X-ray photoelectron spectroscopy suggested formation of the HA layers on the gold surfaces through spontaneous S-Au interaction, resulting in stability in aqueous culture medium. Biological functions of the HA layers were investigated by cell adhesion assay using mouse fibroblast cells (NIH-3T3). Cell attachment to the hydrophilic HA surface (water contact angle ≈ 11°) was far superior to cell attachment to a sugar-free, hydrophobic gold substrate (≈57°), while a spin-coated film composed of water-soluble, TSC-free HA molecules made little contribution to cell adhesion. After 48 h incubation, the NIH-3T3 cells proliferated well only on the HA layers and retained their original viability. This novel approach to surface glyco-modification with biocompatible HA is expected to have potential applications as a biofunctional scaffold for cell culture applications.
AB - Hyaluronan (HA)-fixed layers were successfully prepared on a gold coated glass plate via self-assembly chemisorption of HA derivatives whose aldehydic reducing ends were selectively modified with thiosemicarbazide (TSC). Quartz crystal microbalance analysis and X-ray photoelectron spectroscopy suggested formation of the HA layers on the gold surfaces through spontaneous S-Au interaction, resulting in stability in aqueous culture medium. Biological functions of the HA layers were investigated by cell adhesion assay using mouse fibroblast cells (NIH-3T3). Cell attachment to the hydrophilic HA surface (water contact angle ≈ 11°) was far superior to cell attachment to a sugar-free, hydrophobic gold substrate (≈57°), while a spin-coated film composed of water-soluble, TSC-free HA molecules made little contribution to cell adhesion. After 48 h incubation, the NIH-3T3 cells proliferated well only on the HA layers and retained their original viability. This novel approach to surface glyco-modification with biocompatible HA is expected to have potential applications as a biofunctional scaffold for cell culture applications.
UR - http://www.scopus.com/inward/record.url?scp=77953915072&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953915072&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2010.04.028
DO - 10.1016/j.carbpol.2010.04.028
M3 - Article
AN - SCOPUS:77953915072
VL - 82
SP - 100
EP - 105
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
IS - 1
ER -