Self-induced metabolic state switching by a tunable cell density sensor for microbial isopropanol production

研究成果: ジャーナルへの寄稿記事

46 引用 (Scopus)

抄録

Chemicals production by engineered microorganisms often requires induction of target gene expression at an appropriate cell density to reduce conflict with cell growth. The lux system in Vibrio fischeri is a well-characterized model for cell density-dependent regulation of gene expression termed quorum sensing (QS). However, there are currently no reports for application of the lux system to microbial chemical production. Here, we constructed a synthetic lux system as a tunable cell density sensor-regulator using a synthetic lux promoter and a positive feedback loop in Escherichia coli. In this system, self-induction of a target gene expression is driven by QS-signal, and its threshold cell density can be changed depending on the concentration of a chemical inducer. We demonstrate auto-redirection of metabolic flux from central metabolic pathways toward a synthetic isopropanol pathway at a desired cell density resulting in a significant increase in isopropanol production.

元の言語英語
ページ(範囲)7-15
ページ数9
ジャーナルMetabolic Engineering
30
DOI
出版物ステータス出版済み - 7 1 2015

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2-Propanol
Gene expression
Cell Count
Sensors
Quorum Sensing
Cell growth
Microorganisms
Escherichia coli
Aliivibrio fischeri
Gene Expression
Fluxes
Feedback
Gene Expression Regulation
Metabolic Networks and Pathways
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

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