Second-harmonic generation coherent interferometry was applied to the characterization of the adsorbed layer of a hemicyanine derivative 4-[4-(dimethylamino)styryl]-l-methylpyridinium iodide (DSM) on a fused silica plate. It was clarified that the DSM molecules on the substrate were self-aligned with their methylpyridinium group facing towards the hydrophilic silica substrate.
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