Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells

Naohisa Wada, Hidefumi Maeda, Daigaku Hasegawa, Stan Gronthos, Peter Mark Bartold, Danijela Menicanin, Shinsuke Fujii, Shinichiro Yoshida, Atsushi Tomokiyo, Satoshi Monnouchi, Akifumi Akamine

研究成果: ジャーナルへの寄稿記事

15 引用 (Scopus)

抄録

Periodontal ligament stem cells (PDLSCs) have recently been proposed as a novel option in periodontal regenerative therapy. However, one of the issues is the difficulty of stably generating PDLSCs because of the variation of stem cell potential between donors. Here, we show that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properties in human periodontal ligament (PDL) cells. Sema3A expression was specifically observed in the dental follicle during tooth development and in parts of mature PDL tissue in rodent tooth and periodontal tissue. Sema3A expression levels were found to be higher in multipotential human PDL cell clones compared with low-differentiation potential clones. Sema3A-overexpressing PDL cells exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes. Moreover, PDL cells treated with Sema3A only at the initiation of culture stimulated osteogenesis, while Sema3A treatment throughout the culture had no effect on osteogenic differentiation. Finally, Sema3A-overexpressing PDL cells upregulated the expression of embryonic stem cell markers (NANOG, OCT4, and E-cadherin) and mesenchymal stem cell markers (CD73, CD90, CD105, CD146, and CD166), and Sema3A promoted cell division activity of PDL cells. These results suggest that Sema3A may possess the function to convert PDL cells into mesenchymal-stem-like cells.

元の言語英語
ページ(範囲)2225-2236
ページ数12
ジャーナルStem Cells and Development
23
発行部数18
DOI
出版物ステータス出版済み - 9 15 2014

Fingerprint

Semaphorin-3A
Periodontal Ligament
Stem Cells
Mesenchymal Stromal Cells
Tooth
Dental Sac
Clone Cells
Cadherins
Embryonic Stem Cells
Osteoblasts
Adipocytes
Osteogenesis
Cell Division
Rodentia

All Science Journal Classification (ASJC) codes

  • Hematology
  • Developmental Biology
  • Cell Biology

これを引用

Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. / Wada, Naohisa; Maeda, Hidefumi; Hasegawa, Daigaku; Gronthos, Stan; Bartold, Peter Mark; Menicanin, Danijela; Fujii, Shinsuke; Yoshida, Shinichiro; Tomokiyo, Atsushi; Monnouchi, Satoshi; Akamine, Akifumi.

:: Stem Cells and Development, 巻 23, 番号 18, 15.09.2014, p. 2225-2236.

研究成果: ジャーナルへの寄稿記事

Wada, Naohisa ; Maeda, Hidefumi ; Hasegawa, Daigaku ; Gronthos, Stan ; Bartold, Peter Mark ; Menicanin, Danijela ; Fujii, Shinsuke ; Yoshida, Shinichiro ; Tomokiyo, Atsushi ; Monnouchi, Satoshi ; Akamine, Akifumi. / Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells. :: Stem Cells and Development. 2014 ; 巻 23, 番号 18. pp. 2225-2236.
@article{09cab8f18d394e5fb9779cda586a9af8,
title = "Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells",
abstract = "Periodontal ligament stem cells (PDLSCs) have recently been proposed as a novel option in periodontal regenerative therapy. However, one of the issues is the difficulty of stably generating PDLSCs because of the variation of stem cell potential between donors. Here, we show that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properties in human periodontal ligament (PDL) cells. Sema3A expression was specifically observed in the dental follicle during tooth development and in parts of mature PDL tissue in rodent tooth and periodontal tissue. Sema3A expression levels were found to be higher in multipotential human PDL cell clones compared with low-differentiation potential clones. Sema3A-overexpressing PDL cells exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes. Moreover, PDL cells treated with Sema3A only at the initiation of culture stimulated osteogenesis, while Sema3A treatment throughout the culture had no effect on osteogenic differentiation. Finally, Sema3A-overexpressing PDL cells upregulated the expression of embryonic stem cell markers (NANOG, OCT4, and E-cadherin) and mesenchymal stem cell markers (CD73, CD90, CD105, CD146, and CD166), and Sema3A promoted cell division activity of PDL cells. These results suggest that Sema3A may possess the function to convert PDL cells into mesenchymal-stem-like cells.",
author = "Naohisa Wada and Hidefumi Maeda and Daigaku Hasegawa and Stan Gronthos and Bartold, {Peter Mark} and Danijela Menicanin and Shinsuke Fujii and Shinichiro Yoshida and Atsushi Tomokiyo and Satoshi Monnouchi and Akifumi Akamine",
year = "2014",
month = "9",
day = "15",
doi = "10.1089/scd.2013.0405",
language = "English",
volume = "23",
pages = "2225--2236",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary Ann Liebert Inc.",
number = "18",

}

TY - JOUR

T1 - Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells

AU - Wada, Naohisa

AU - Maeda, Hidefumi

AU - Hasegawa, Daigaku

AU - Gronthos, Stan

AU - Bartold, Peter Mark

AU - Menicanin, Danijela

AU - Fujii, Shinsuke

AU - Yoshida, Shinichiro

AU - Tomokiyo, Atsushi

AU - Monnouchi, Satoshi

AU - Akamine, Akifumi

PY - 2014/9/15

Y1 - 2014/9/15

N2 - Periodontal ligament stem cells (PDLSCs) have recently been proposed as a novel option in periodontal regenerative therapy. However, one of the issues is the difficulty of stably generating PDLSCs because of the variation of stem cell potential between donors. Here, we show that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properties in human periodontal ligament (PDL) cells. Sema3A expression was specifically observed in the dental follicle during tooth development and in parts of mature PDL tissue in rodent tooth and periodontal tissue. Sema3A expression levels were found to be higher in multipotential human PDL cell clones compared with low-differentiation potential clones. Sema3A-overexpressing PDL cells exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes. Moreover, PDL cells treated with Sema3A only at the initiation of culture stimulated osteogenesis, while Sema3A treatment throughout the culture had no effect on osteogenic differentiation. Finally, Sema3A-overexpressing PDL cells upregulated the expression of embryonic stem cell markers (NANOG, OCT4, and E-cadherin) and mesenchymal stem cell markers (CD73, CD90, CD105, CD146, and CD166), and Sema3A promoted cell division activity of PDL cells. These results suggest that Sema3A may possess the function to convert PDL cells into mesenchymal-stem-like cells.

AB - Periodontal ligament stem cells (PDLSCs) have recently been proposed as a novel option in periodontal regenerative therapy. However, one of the issues is the difficulty of stably generating PDLSCs because of the variation of stem cell potential between donors. Here, we show that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properties in human periodontal ligament (PDL) cells. Sema3A expression was specifically observed in the dental follicle during tooth development and in parts of mature PDL tissue in rodent tooth and periodontal tissue. Sema3A expression levels were found to be higher in multipotential human PDL cell clones compared with low-differentiation potential clones. Sema3A-overexpressing PDL cells exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes. Moreover, PDL cells treated with Sema3A only at the initiation of culture stimulated osteogenesis, while Sema3A treatment throughout the culture had no effect on osteogenic differentiation. Finally, Sema3A-overexpressing PDL cells upregulated the expression of embryonic stem cell markers (NANOG, OCT4, and E-cadherin) and mesenchymal stem cell markers (CD73, CD90, CD105, CD146, and CD166), and Sema3A promoted cell division activity of PDL cells. These results suggest that Sema3A may possess the function to convert PDL cells into mesenchymal-stem-like cells.

UR - http://www.scopus.com/inward/record.url?scp=84906991645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906991645&partnerID=8YFLogxK

U2 - 10.1089/scd.2013.0405

DO - 10.1089/scd.2013.0405

M3 - Article

C2 - 24380401

AN - SCOPUS:84906991645

VL - 23

SP - 2225

EP - 2236

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 18

ER -