Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement

Michihiko Nakano, Hiromichi Kasahara, Zhenhao Ding, Junya Suehiro

研究成果: 著書/レポートタイプへの貢献会議での発言

抄録

We proposed that a rapid detection method of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads. This electrical method measures the impedance change of the DEP trapped microbeads on which target DNA molecules are chemically attached. The method realizes easy and rapid DNA detection after conventional PCR. In this study, it was demonstrated that quantitative and high sensitivity detection of virus genome using the method as comparing with real-time PCR. Real-time PCR detects target DNA sensitive and quantitative by real-time monitoring of DNA amplification. As the results, the DNA detection method using microbeads DEP showed the same detection limit as the real-time PCR, and demonstrated the quantitative detection in the range of 101 to 105 copies/reaction. Our new method can realize inexpensive and rapid PCR-based viral detection by combined with conventional PCR.

元の言語英語
ホスト出版物のタイトル2015 IEEE SENSORS - Proceedings
出版者Institute of Electrical and Electronics Engineers Inc.
ISBN(電子版)9781479982028
DOI
出版物ステータス出版済み - 12 31 2015
イベント14th IEEE SENSORS - Busan, 大韓民国
継続期間: 11 1 201511 4 2015

出版物シリーズ

名前2015 IEEE SENSORS - Proceedings

その他

その他14th IEEE SENSORS
大韓民国
Busan
期間11/1/1511/4/15

Fingerprint

polymerase chain reaction
Polymerase chain reaction
impedance measurement
beads
DNA
deoxyribonucleic acid
Electrophoresis
genome
viruses
Viruses
Amplification
Genes
impedance
Molecules
Monitoring
sensitivity

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electronic, Optical and Magnetic Materials
  • Spectroscopy
  • Electrical and Electronic Engineering

これを引用

Nakano, M., Kasahara, H., Ding, Z., & Suehiro, J. (2015). Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement. : 2015 IEEE SENSORS - Proceedings [7370216] (2015 IEEE SENSORS - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2015.7370216

Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement. / Nakano, Michihiko; Kasahara, Hiromichi; Ding, Zhenhao; Suehiro, Junya.

2015 IEEE SENSORS - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2015. 7370216 (2015 IEEE SENSORS - Proceedings).

研究成果: 著書/レポートタイプへの貢献会議での発言

Nakano, M, Kasahara, H, Ding, Z & Suehiro, J 2015, Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement. : 2015 IEEE SENSORS - Proceedings., 7370216, 2015 IEEE SENSORS - Proceedings, Institute of Electrical and Electronics Engineers Inc., 14th IEEE SENSORS, Busan, 大韓民国, 11/1/15. https://doi.org/10.1109/ICSENS.2015.7370216
Nakano M, Kasahara H, Ding Z, Suehiro J. Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement. : 2015 IEEE SENSORS - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2015. 7370216. (2015 IEEE SENSORS - Proceedings). https://doi.org/10.1109/ICSENS.2015.7370216
Nakano, Michihiko ; Kasahara, Hiromichi ; Ding, Zhenhao ; Suehiro, Junya. / Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement. 2015 IEEE SENSORS - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2015. (2015 IEEE SENSORS - Proceedings).
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