TY - GEN
T1 - Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement
AU - Nakano, Michihiko
AU - Kasahara, Hiromichi
AU - Ding, Zhenhao
AU - Suehiro, Junya
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/12/31
Y1 - 2015/12/31
N2 - We proposed that a rapid detection method of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads. This electrical method measures the impedance change of the DEP trapped microbeads on which target DNA molecules are chemically attached. The method realizes easy and rapid DNA detection after conventional PCR. In this study, it was demonstrated that quantitative and high sensitivity detection of virus genome using the method as comparing with real-time PCR. Real-time PCR detects target DNA sensitive and quantitative by real-time monitoring of DNA amplification. As the results, the DNA detection method using microbeads DEP showed the same detection limit as the real-time PCR, and demonstrated the quantitative detection in the range of 101 to 105 copies/reaction. Our new method can realize inexpensive and rapid PCR-based viral detection by combined with conventional PCR.
AB - We proposed that a rapid detection method of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads. This electrical method measures the impedance change of the DEP trapped microbeads on which target DNA molecules are chemically attached. The method realizes easy and rapid DNA detection after conventional PCR. In this study, it was demonstrated that quantitative and high sensitivity detection of virus genome using the method as comparing with real-time PCR. Real-time PCR detects target DNA sensitive and quantitative by real-time monitoring of DNA amplification. As the results, the DNA detection method using microbeads DEP showed the same detection limit as the real-time PCR, and demonstrated the quantitative detection in the range of 101 to 105 copies/reaction. Our new method can realize inexpensive and rapid PCR-based viral detection by combined with conventional PCR.
UR - http://www.scopus.com/inward/record.url?scp=84963525919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84963525919&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2015.7370216
DO - 10.1109/ICSENS.2015.7370216
M3 - Conference contribution
AN - SCOPUS:84963525919
T3 - 2015 IEEE SENSORS - Proceedings
BT - 2015 IEEE SENSORS - Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 14th IEEE SENSORS
Y2 - 1 November 2015 through 4 November 2015
ER -