Sensitive detection of fluorescence in western blotting by merging images

Yukari Kondo, Shinichiro Higa, Takeshi Iwasaki, Tomoya Matsumoto, Kazumitsu Maehara, Akihito Harada, Yoshihiro Baba, Masatoshi Fujita, Yasuyuki Ohkawa

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

The western blotting technique is widely used to analyze protein expression levels and protein molecular weight. The chemiluminescence method is mainly used for detection due to its high sensitivity and ease of manipulation, but it is unsuitable for detailed analyses because it cannot be used to detect multiple proteins simultaneously. Recently, more attention has been paid to the fluorescence detection method because it is more quantitative and is suitable for the detection of multiple proteins simultaneously. However, fluorescence detection can be limited by poor image resolution and low detection sensitivity. Here, we describe a method to detect fluorescence in western blots using fluorescence microscopy to obtain high-resolution images. In this method, filters and fluorescent dyes are optimized to enhance detection sensitivity to a level similar to that of the chemiluminescence method.

元の言語英語
記事番号e0191532
ジャーナルPloS one
13
発行部数1
DOI
出版物ステータス出版済み - 1 1 2018

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Merging
Western blotting
Fluorescence
Western Blotting
fluorescence
Chemiluminescence
Image resolution
Luminescence
Proteins
chemiluminescence
Fluorescence microscopy
methodology
Fluorescent Dyes
Fluorescence Microscopy
proteins
fluorescent dyes
Molecular weight
fluorescence microscopy
Molecular Weight
protein synthesis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Sensitive detection of fluorescence in western blotting by merging images. / Kondo, Yukari; Higa, Shinichiro; Iwasaki, Takeshi; Matsumoto, Tomoya; Maehara, Kazumitsu; Harada, Akihito; Baba, Yoshihiro; Fujita, Masatoshi; Ohkawa, Yasuyuki.

:: PloS one, 巻 13, 番号 1, e0191532, 01.01.2018.

研究成果: ジャーナルへの寄稿記事

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AU - Higa, Shinichiro

AU - Iwasaki, Takeshi

AU - Matsumoto, Tomoya

AU - Maehara, Kazumitsu

AU - Harada, Akihito

AU - Baba, Yoshihiro

AU - Fujita, Masatoshi

AU - Ohkawa, Yasuyuki

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