Sensitive determination of pentazocine in human tissues by high-performance liquid chromatography

Tohru Imamura, Keiko Kudo, Akira Namera, Mikio Yashiki, Tohru Kojima

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

The authors developed a simple, reliable and sensitive method for the determination of pentazocine in human solid tissues using high-performance liquid chromatography, combined with a three-step liquid-liquid extraction procedure. Levallorphan tartrate served as the internal standard. The extract was evaporated to dryness and dissolved in the mobile phase of acetonitrile/10 mM phosphate buffer (pH 4.0). The eluent was pumped at a flow rate of 0.4 ml/min through a Spherisorb(R) Ph (2.1 mm I.D. x 150 mm) column. A fluorescence detector with excitation at 247 nm and emission at 320 nm was used. The lower limit of detection was about 0.5 ng/g. The calibration curve was linear over the concentration ranges from 1 to 500 ng/g in each tissue examined and could be determined up to at least 10.0 μg/g by means of reduction of injected volumes. Using this method, the concentrations of pentazocine could be determined in the tissues of an autopsied individual for toxicological evaluation.

本文言語英語
ページ(範囲)119-122
ページ数4
ジャーナルLegal Medicine
2
2
DOI
出版ステータス出版済み - 2000

All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 諸問題の倫理的、法的側面

フィンガープリント

「Sensitive determination of pentazocine in human tissues by high-performance liquid chromatography」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル