Sensitive determination of sulpiride in human plasma by high-performance liquid chromatography

H. Tokunaga, Keiko Kudo, N. Jitsufuchi, Y. Ohtsuka, T. Imamura

研究成果: ジャーナルへの寄稿記事

40 引用 (Scopus)

抄録

We developed a simple, sensitive and reliable method for the determination of sulpiride, a specific antipsychotic drug, in human plasma using high-performance liquid chromatography. A structurally related benzamide, tiapride, was used as the internal standard. A Sep-Pak C18 cartridge was used to extract a sample from 1 ml of plasma. The extract was dissolved in methylene chloride, and then back-extracted with 0.01 M hydrochloric acid. The aqueous layer was put on a octadecylsilica column with a mobile phase of 50% acetonitrile in 0.01 M phosphate buffer (pH 3.0). A fluorescence detector with excitation at 300 nm and emission at 365 nm was used for detection. The calibration curve was linear in the concentration range of 10-1500 ng/ml, and the lower limit of detection was 1 ng/ml. We used this method to examine plasma levels of sulpiride in 14 inpatients being treated with sulpiride for 6 months. The determined plasma levels were 70.1-1121.2 ng/ml, and the correlation between daily dose and plasma concentration was positive. This simple, reliable method is expected to be put to good use in forensic and hospital laboratories.

元の言語英語
ページ(範囲)203-207
ページ数5
ジャーナルJournal of Chromatography B: Biomedical Applications
691
発行部数1
DOI
出版物ステータス出版済み - 3 28 1997

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Plasma (human)
Sulpiride
High performance liquid chromatography
Plasmas
Tiapride Hydrochloride
Hydrochloric Acid
Methylene Chloride
Antipsychotic Agents
Buffers
Fluorescence
Phosphates
Calibration
Detectors

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

これを引用

Sensitive determination of sulpiride in human plasma by high-performance liquid chromatography. / Tokunaga, H.; Kudo, Keiko; Jitsufuchi, N.; Ohtsuka, Y.; Imamura, T.

:: Journal of Chromatography B: Biomedical Applications, 巻 691, 番号 1, 28.03.1997, p. 203-207.

研究成果: ジャーナルへの寄稿記事

Tokunaga, H. ; Kudo, Keiko ; Jitsufuchi, N. ; Ohtsuka, Y. ; Imamura, T. / Sensitive determination of sulpiride in human plasma by high-performance liquid chromatography. :: Journal of Chromatography B: Biomedical Applications. 1997 ; 巻 691, 番号 1. pp. 203-207.
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