Sensitive determination of tetrodotoxin in human plasma by gas chromatography/mass spectrometry

Yukio Ohtsuka, Keiko Kudo, Sunao Fukushima, Akiko Tsuji, Noriaki Ikeda

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

A sensitive and reliable method for determination of tetrodotoxin (TTX) in human plasma by gas chromatography/ mass spectrometry (GC/MS) was developed. TTX was extracted from plasma with 2% acetic acid in a mixture of methanol and acetonitrile (8:2), passed through the first cartridge, and then transformed into 2-amino-6-hydroxymethyl-8-hydroxyquinazoline (C9-base) with 1 M potassium hydroxide. The C9-base was purified with the second cartridge, converted to the TMS derivative together with β-estradiol (IS) and then applied to a GC/MS instrument. Three kinds of cartridges, Sep-Pak C18, Sep-Pak tC18 and Sep-Pak PS-2, were all found to be equally useful when used as the first cartridge, but the Sep-Pak PS-2 gave the best recovery when used as the second cartridge. The method described here enabled the quantitation of TTX in human plasma in the range of 0.5-100 ng/ml with the lowest limit of detection of 0.5 ng/ml.

本文言語英語
ページ(範囲)188-192
ページ数5
ジャーナルJapanese Journal of Forensic Toxicology
22
3
出版ステータス出版済み - 2004

!!!All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 毒物学

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