Separation of Lipids

T. Yamada, K. Taguchi, Takeshi Bamba

研究成果: Chapter in Book/Report/Conference proceedingChapter

6 被引用数 (Scopus)

抄録

Supercritical fluid chromatography (SFC) is one of the useful techniques used in lipid analysis. Since lipids comprise an enormous number of molecular species with similar structures, high-resolution chromatographic separation, coupled with mass spectrometry (MS) or other detection methods, is required for detailed profiling of molecular species. The high diffusivity and low polarity of supercritical carbon dioxide (SCCO2) enables the separation of individual lipid molecular species with good resolution. The low viscosity of SCCO2 allows for a high flow rate of the SFC mobile phase, resulting in shorter separation time compared with liquid chromatography (LC). In addition, polar lipids can be analyzed using gradient elution with SCCO2 and a modifier.Supercritical fluid extraction (SFE) can be combined directly with SFC, enabling high-throughput analysis of thermally labile and oxidizable hydrophobic compounds. These supercritical fluid-related techniques have potential applications in biomarker discovery and elucidation of the detailed mechanisms of lipid metabolism.

本文言語英語
ホスト出版物のタイトルSupercritical Fluid Chromatography
ホスト出版物のサブタイトルHandbooks in Separation Science
出版社Elsevier Inc.
ページ419-438
ページ数20
ISBN(電子版)9780128093672
ISBN(印刷版)9780128092071
DOI
出版ステータス出版済み - 2 20 2017

All Science Journal Classification (ASJC) codes

  • 化学 (全般)

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