Separation of non-esterified chlorophyls by ion-suppression high-performance liquid chromatography

Yuzo Shioi, Michio Doi, Tsutomu Sasa

研究成果: Contribution to journalArticle査読

23 被引用数 (Scopus)

抄録

Chlorophyllides and pheophorbides were separated by ion-suppression high-performance liquid chromatography using an octadecyl silica column eluted with 80-95% (v/v) methanol in water containing 13 mM acetic (final pH 4.2) as the suppessing ion. The separated pigments were detected fluorometrically with a sensitivity in the picomol range without artifact formation. This technique can be used not only for the determination of non-esterified chlorophylls, but also for esterified chlorophylls, thus enabling the simultaneous identification of chlorophylls and their derivatives.

本文言語英語
ページ(範囲)141-149
ページ数9
ジャーナルJournal of Chromatography A
298
C
DOI
出版ステータス出版済み - 1984

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 有機化学

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