Separation of transmitted light and scattering components in transmitted microscopy

Mihoko Shimano, Ryoma Bise, Yinqiang Zheng, Imari Sato

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

3 被引用数 (Scopus)

抄録

In transmitted light microscopy, a specimen tends to be observed as unclear. This is caused by a phenomenon that an image sensor captures the sum of these scattered light rays traveled from different paths due to scattering. To cope with this problem, we propose a novel computational photography approach for separating directly transmitted light from the scattering light in a transmitted light microscope by using high-frequency lighting. We first investigated light paths and clarified what types of light overlap in transmitted light microscopy. The scattered light can be simply represented and removed by using the difference in observations between focused and unfocused conditions, where the high-frequency illumination becomes homogeneous. Our method makes a novel spatial multiple-spectral absorption analysis possible, which requires absorption coefficients to be measured in each spectrum at each position. Experiments on real biological tissues demonstrated the effectiveness of our method.

本文言語英語
ホスト出版物のタイトルMedical Image Computing and Computer Assisted Intervention − MICCAI 2017 - 20th International Conference, Proceedings
編集者Pierre Jannin, Simon Duchesne, Maxime Descoteaux, Alfred Franz, D. Louis Collins, Lena Maier-Hein
出版社Springer Verlag
ページ12-20
ページ数9
ISBN(印刷版)9783319661841
DOI
出版ステータス出版済み - 2017
イベント20th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2017 - Quebec City, カナダ
継続期間: 9 11 20179 13 2017

出版物シリーズ

名前Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
10434 LNCS
ISSN(印刷版)0302-9743
ISSN(電子版)1611-3349

その他

その他20th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2017
国/地域カナダ
CityQuebec City
Period9/11/179/13/17

All Science Journal Classification (ASJC) codes

  • 理論的コンピュータサイエンス
  • コンピュータ サイエンス(全般)

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