Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus

A. Iwasaki, Y. Yoshikai, M. Sakumoto, K. Himeno, H. Yuuki, M. Kumamoto, K. Sueishi, K. Nomoto

    研究成果: Contribution to journalArticle査読

    14 被引用数 (Scopus)

    抄録

    To elucidate the abnormality of T cell differentiation in nude mice grafted with rat fetal thymus that develop multiple-organ-localized autoimmune diseases, we examined sequential appearance of T cell subsets and expression of TCR genes in BALB/c nude mice after grafting with fetal F344 rat thymus. We observed progressive expression of TCRγ/δ-α/β genes in the lymph node (LN) cells from 8 to 12 wk after grafting. An appreciable number of CD4+ T cells but few CD8+ T cells were detected in the LN at 8 wk after grafting. CD8+ T cells increased slowly in number by 12 wk after grafting but remained at a low level in comparison with those in nude mice 12 wk after grafting with BALB/c thymus. In correlation with an increase in the number of T cells expressing TCR α/β genes, alloreactivity as assessed by MLR was increased to a normal level. However, CTL activity against alloantigens remained at a low level in the LN cells at 12 wk. At this stage, organ-specific autoimmune diseases and a high level of anti-DNA autoantibodies were detected. In these mice host-reactive T cells such as Vβ3- or Vβ11-bearing T cells were virtually eliminated in the peripheral mature T cell pool, whereas T cells maturing in the fetal rat thymus significantly proliferated in response to donor-rat stimulator cells. These results suggest that the development of the autoimmune diseases may be ascribed to an impaired maturation of CD8+ T cells but not to failure in clonal elimination of host-reactive T cells in nude mice grafted with rat thymus.

    本文言語英語
    ページ(範囲)28-35
    ページ数8
    ジャーナルJournal of Immunology
    145
    1
    出版ステータス出版済み - 1 1 1990

    All Science Journal Classification (ASJC) codes

    • 免疫アレルギー学
    • 免疫学

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