Sequential conversion of cortexolone to prednisolone by immobilized mycelia of Curvularia lunata and immobilized cells of Arthrobacter simplex

Tapan Kumar Mazumder, Kenji Sonomoto, Atsuo Tanaka, Saburo Fukui

研究成果: ジャーナルへの寄稿記事

31 引用 (Scopus)

抄録

Two-step bioconversion of cortexolone (Reichstein's Compound S) to its Δ1-dehaydro-11 β-hydroxy derivative, prednisolone, was successfully performed by the combined use of immobilized Curvularia lunata mycelia and immobilized Arthrobacter simplex cells. Immobilized living mycelia of C. lunata having a high 11β-hydroxylation activity were prepared by in situ germination of spores entrapped in photo-crosslinked resin gels of a suitable net-work structure. Acetone-dried cells of A. simplex having an induced steroid Δ1-dehydrogenase activity were also entrapped with photo-crosslinkable resin prepolymers and used for Δ- dehydrogenation of hydrocortisone to prednisolone. For the production of prednisolone from cortexolone, the combination of sequential steps, 11β-hydroxylation and subsequent Δ1-dehydrogenation, was found to be suitable. Each immobilized microbial cell system was stable and could be used for the sequential reactions repeatedly (operational period, 25 days).

元の言語英語
ページ(範囲)154-161
ページ数8
ジャーナルApplied Microbiology and Biotechnology
21
発行部数3-4
DOI
出版物ステータス出版済み - 2 1 1985
外部発表Yes

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Cortodoxone
Arthrobacter
Immobilized Cells
Mycelium
Prednisolone
3-oxosteroid delta(1) dehydrogenase
Hydroxylation
Germination
Acetone
Spores
Hydrocortisone
Gels

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

これを引用

Sequential conversion of cortexolone to prednisolone by immobilized mycelia of Curvularia lunata and immobilized cells of Arthrobacter simplex. / Mazumder, Tapan Kumar; Sonomoto, Kenji; Tanaka, Atsuo; Fukui, Saburo.

:: Applied Microbiology and Biotechnology, 巻 21, 番号 3-4, 01.02.1985, p. 154-161.

研究成果: ジャーナルへの寄稿記事

Mazumder, Tapan Kumar ; Sonomoto, Kenji ; Tanaka, Atsuo ; Fukui, Saburo. / Sequential conversion of cortexolone to prednisolone by immobilized mycelia of Curvularia lunata and immobilized cells of Arthrobacter simplex. :: Applied Microbiology and Biotechnology. 1985 ; 巻 21, 番号 3-4. pp. 154-161.
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abstract = "Two-step bioconversion of cortexolone (Reichstein's Compound S) to its Δ1-dehaydro-11 β-hydroxy derivative, prednisolone, was successfully performed by the combined use of immobilized Curvularia lunata mycelia and immobilized Arthrobacter simplex cells. Immobilized living mycelia of C. lunata having a high 11β-hydroxylation activity were prepared by in situ germination of spores entrapped in photo-crosslinked resin gels of a suitable net-work structure. Acetone-dried cells of A. simplex having an induced steroid Δ1-dehydrogenase activity were also entrapped with photo-crosslinkable resin prepolymers and used for Δ- dehydrogenation of hydrocortisone to prednisolone. For the production of prednisolone from cortexolone, the combination of sequential steps, 11β-hydroxylation and subsequent Δ1-dehydrogenation, was found to be suitable. Each immobilized microbial cell system was stable and could be used for the sequential reactions repeatedly (operational period, 25 days).",
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