Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin

Toshiki Tsurimoto, Thomas Melendy, Bruce Stillman

研究成果: Contribution to journalArticle査読

316 被引用数 (Scopus)

抄録

Enzymatic synthesis of DNA from the simian virus 40 origin of DNA replication has been reconstituted in vitro with eight purified components. DNA polymerase α-primase complex first initiates DNA synthesis at the replication origin and continues as the lagging strand polymerase. Subsequently, the DNA polymerase δ complex initiates replication on the leading strand template. Some prokaryotic DNA polymerase complexes can replace the eukaryotic polymerase δ complex. A model for polymerase switching during initiation of DNA replication is presented.

本文言語英語
ページ(範囲)534-539
ページ数6
ジャーナルNature
346
6284
DOI
出版ステータス出版済み - 1 1 1990
外部発表はい

All Science Journal Classification (ASJC) codes

  • 一般

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