Sequential injection immunoassay for environmental measurements

Nobuaki Soh, Mayumi Tanaka, Koji Hirakawa, Rui Qi Zhang, Hizuru Nakajima, Koji Nakano, Toshihiko Imato

    研究成果: ジャーナルへの寄稿学術誌査読

    7 被引用数 (Scopus)


    Sequential injection immunoassay systems for environmental measurements based on the selective immunoreaction between antigen and antibody were described. A sequential injection analysis (SIA) technique is suitable to be applied for the procedure of enzyme-linked immunosorbent assay (ELISA), because the washing and the addition of reagent solutions can be automated by using a computer-controlled syringe pump and switching valve. We selected vitellogenin (Vg), which is a biomarker for evaluating environmental risk caused by endocrine-disrupting chemicals in the hydrosphere, and linear alkylbenzene sulfonates (LAS) and alkylphenol polyethoxylates (APEO), which are versatile surfactants, as target analytes in the flow immunoassay systems. For Vg monitoring, SIA systems based on spectrophotometric, chemiluminescence, and electrochemical determinations were constructed. On the other hand, chemiluminescence determination was applied to the detection of LAS and APEO. For APEO, an SIA system combined with surface plasmon resonance (SPR) sensor was also developed. These new sequential injection immunoassay systems are expected to be useful systems for environmental analysis.

    ジャーナルanalytical sciences
    出版ステータス出版済み - 2011

    !!!All Science Journal Classification (ASJC) codes

    • 分析化学


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