Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilter

Mitsuhiko Onda, Yuri Lvov, Katsuhiko Ariga, Toyoki Kunitake

研究成果: ジャーナルへの寄稿学術誌査読

196 被引用数 (Scopus)

抄録

Separate molecular layers of glucoamylase (GA) and glucose oxidase (GOD) were assembled on an ultrafilter by layer-by-layer adsorption using poly(ethyleneimine) (PEI) as a polycation. An aqueous starch solution was placed on the ultrafilter which was covered by GA and GOD layers in various sequential arrangements with intervening polyion complex layers of PEI and poly(styrenesulfonate) (PSS). GA hydrolyzed starch to glucose, which was then converted to gluconic acid and H2O2 by GOD. The films with an upper GA layer and a lower GOD layer showed higher overall activities, consistent with the order of the sequential enzymatic reactions. Reaction products and unreacted starch were readily separated by filtration when the substrate solution was passed through these multi-enzyme films.

本文言語英語
ページ(範囲)502-506
ページ数5
ジャーナルJournal of Fermentation and Bioengineering
82
5
DOI
出版ステータス出版済み - 1996

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

フィンガープリント

「Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilter」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル