TY - JOUR
T1 - Serum factors from patients with systemic lupus erythematosus enhancing superoxide generation by normal neutrophils
AU - Shingu, M.
AU - Oribe, M.
AU - Todoroki, T.
AU - Tatsukawa, K.
AU - Tomo-oka, K.
AU - Yasuda, M.
AU - Nobunaga, M.
PY - 1983
Y1 - 1983
N2 - It has been suggested that human neutrophils exposed to preformed immune complexes or activated complement fragments generate O2- anions in extracellular medium. In vivo studies have revealed that oxygen intermediates produced by immune complex-activated neutrophils play an important role in subsequent tissue damage. Since it is difficult to obtain direct evidence that O2- is released into plasma in patients with systemic lupus erythematosus (SLE), we studied the capacities of their sera to stimulate O2- release by human neutrophils in vitro. Sera from patients with SLE significantly enhanced O2- generation by neutrophils compared to normal sera. The enhancing activity of serum in the induction of increased O2- generation correlated positively with the presence of serum immune complexes and negatively with serum complement levels. The enhancing factors were analyzed by serum fractionation on Sephadex G-200 gel filtration, and were concluded to be immune complexes of intermediate size containing an activated complement fragment.
AB - It has been suggested that human neutrophils exposed to preformed immune complexes or activated complement fragments generate O2- anions in extracellular medium. In vivo studies have revealed that oxygen intermediates produced by immune complex-activated neutrophils play an important role in subsequent tissue damage. Since it is difficult to obtain direct evidence that O2- is released into plasma in patients with systemic lupus erythematosus (SLE), we studied the capacities of their sera to stimulate O2- release by human neutrophils in vitro. Sera from patients with SLE significantly enhanced O2- generation by neutrophils compared to normal sera. The enhancing activity of serum in the induction of increased O2- generation correlated positively with the presence of serum immune complexes and negatively with serum complement levels. The enhancing factors were analyzed by serum fractionation on Sephadex G-200 gel filtration, and were concluded to be immune complexes of intermediate size containing an activated complement fragment.
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U2 - 10.1111/1523-1747.ep12517989
DO - 10.1111/1523-1747.ep12517989
M3 - Article
C2 - 6309985
AN - SCOPUS:0020595085
VL - 81
SP - 212
EP - 215
JO - Quaternary International
JF - Quaternary International
SN - 1040-6182
IS - 3
ER -