Shear-stress effect on mitochondrial membrane potential and albumin uptake in cultured endothelial cells

Susumu Kudo, Ryoma Morigaki, Junichi Saito, Mariko Ikeda, Kotaro Oka, Kazuo Tanishita

研究成果: Contribution to journalArticle査読

28 被引用数 (Scopus)

抄録

Endothelial cells (ECs) that line the inner surface of blood vessels are continuously exposed to shear stress induced by blood flow in vivo and shear stress affects ATP-dependent macromolecular transport in ECs. However, the relationship between the ATP production and shear stress is still unclear. We, therefore, evaluated mitochondrial ATP synthesis activity in cultured endothelial cells exposed to shear stress, using a confocal laser scanning microscope (CLSM) and a mitochondrial membrane potential probe (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolycarbocyanine iodide, JC-1). Low shear stress (10 dyn/cm2) increased mitochondrial membrane potential by 30%. On the contrary, high shear stress (60 dyn/cm2) decreased it by 20%. This observation was consistent with the ATP-dependent albumin uptake into endothelial cells. Our results indicate that ATP synthetic activity is related to the albumin uptake into endothelial cells. (C) 2000 Academic Press.

本文言語英語
ページ(範囲)616-621
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
270
2
DOI
出版ステータス出版済み - 4 13 2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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