TY - CHAP
T1 - Sialidase-Resistant Ganglioside GM3 Analogues
T2 - Evaluation of Biological Activity
AU - Hirai, Go
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Glycolipids play important biological roles mainly in biological membranes, but their functions at the molecular level remain to be fully established. A chemical biology approach using exogenously added glycolipid probes would be promising, but the possibility of cleavage by cellular glycohydrolases complicates the interpretation of results. Thus, there is a need for non-hydolyzable analogues. In the present study, we designed and synthesized GM3 analogues resistant to GM3-degrading sialidase by replacing the O-sialoside linkage with a C-sialoside linkage. The bioactivity of the analogues was also investigated.
AB - Glycolipids play important biological roles mainly in biological membranes, but their functions at the molecular level remain to be fully established. A chemical biology approach using exogenously added glycolipid probes would be promising, but the possibility of cleavage by cellular glycohydrolases complicates the interpretation of results. Thus, there is a need for non-hydolyzable analogues. In the present study, we designed and synthesized GM3 analogues resistant to GM3-degrading sialidase by replacing the O-sialoside linkage with a C-sialoside linkage. The bioactivity of the analogues was also investigated.
UR - http://www.scopus.com/inward/record.url?scp=85145425116&partnerID=8YFLogxK
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U2 - 10.1007/978-1-0716-2910-9_7
DO - 10.1007/978-1-0716-2910-9_7
M3 - Chapter
C2 - 36587072
AN - SCOPUS:85145425116
T3 - Methods in Molecular Biology
SP - 79
EP - 87
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -