Significance of the HLA‐DQB matching in one‐haplotype identical kidney transplant pairs and the matching analysis by the polymerase chain reaction (PCR) — heteroduplex — polymorphism method

Y. Fukuda, A. Kimura, S. Hoshino, H. Tashiro, M. Furukawa, S. Shintaku, H. Hori, Takehiko Sasazuki, K. Dohi

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

Abstract: Sixty‐five living related kidney transplant pairs were analyzed for matching at HLA class II loci by the polymerase chain reaction (PCR)‐ sequence specific oligonucleotide probe (SSOP) method. The retrospective HLA matching study revealed that there were many early graft loss cases despite the DQB compatibility, contrary to our expectation. There were 54 DRB1 one‐mismatched cases, in which 7 of the 11 (64%) DQB zero‐mismatched cases had lost their grafts, while the graft loss cases were only 10 of the 43 (23%) DQB one‐mismatched pairs (P value=0.0006). The DQB matching of these cases was studied in detail, because the PCR‐SSOP methods are based on the detection of sequence polymorphisms in a relatively narrow range, i.e., recognized sequences by SSOPs. The PCR ‐ heteroduplex ‐ polymorphism analysis method was developed to analyze the polymorphism in exon 2 of the DQB1 gene. However, all the pairs proved to be compatible for the DQB, demonstrating that the DQB compatibility was associated with a harmful influence on the graft outcome. These observations suggested that the DQB1 incompatibility might exert the low responsiveness to HLA haplo‐identical allogeneic transplants.

元の言語英語
ページ(範囲)49-56
ページ数8
ジャーナルTissue antigens
45
発行部数1
DOI
出版物ステータス出版済み - 1 1 1995

Fingerprint

Transplants
Polymerase chain reaction
Polymorphism
Grafts
Kidney
Polymerase Chain Reaction
Oligonucleotide Probes
Heteroduplex Analysis
Exons
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

これを引用

Significance of the HLA‐DQB matching in one‐haplotype identical kidney transplant pairs and the matching analysis by the polymerase chain reaction (PCR) — heteroduplex — polymorphism method. / Fukuda, Y.; Kimura, A.; Hoshino, S.; Tashiro, H.; Furukawa, M.; Shintaku, S.; Hori, H.; Sasazuki, Takehiko; Dohi, K.

:: Tissue antigens, 巻 45, 番号 1, 01.01.1995, p. 49-56.

研究成果: ジャーナルへの寄稿記事

Fukuda, Y. ; Kimura, A. ; Hoshino, S. ; Tashiro, H. ; Furukawa, M. ; Shintaku, S. ; Hori, H. ; Sasazuki, Takehiko ; Dohi, K. / Significance of the HLA‐DQB matching in one‐haplotype identical kidney transplant pairs and the matching analysis by the polymerase chain reaction (PCR) — heteroduplex — polymorphism method. :: Tissue antigens. 1995 ; 巻 45, 番号 1. pp. 49-56.
@article{c1539625c12e44d28eda8a728360d219,
title = "Significance of the HLA‐DQB matching in one‐haplotype identical kidney transplant pairs and the matching analysis by the polymerase chain reaction (PCR) — heteroduplex — polymorphism method",
abstract = "Abstract: Sixty‐five living related kidney transplant pairs were analyzed for matching at HLA class II loci by the polymerase chain reaction (PCR)‐ sequence specific oligonucleotide probe (SSOP) method. The retrospective HLA matching study revealed that there were many early graft loss cases despite the DQB compatibility, contrary to our expectation. There were 54 DRB1 one‐mismatched cases, in which 7 of the 11 (64{\%}) DQB zero‐mismatched cases had lost their grafts, while the graft loss cases were only 10 of the 43 (23{\%}) DQB one‐mismatched pairs (P value=0.0006). The DQB matching of these cases was studied in detail, because the PCR‐SSOP methods are based on the detection of sequence polymorphisms in a relatively narrow range, i.e., recognized sequences by SSOPs. The PCR ‐ heteroduplex ‐ polymorphism analysis method was developed to analyze the polymorphism in exon 2 of the DQB1 gene. However, all the pairs proved to be compatible for the DQB, demonstrating that the DQB compatibility was associated with a harmful influence on the graft outcome. These observations suggested that the DQB1 incompatibility might exert the low responsiveness to HLA haplo‐identical allogeneic transplants.",
author = "Y. Fukuda and A. Kimura and S. Hoshino and H. Tashiro and M. Furukawa and S. Shintaku and H. Hori and Takehiko Sasazuki and K. Dohi",
year = "1995",
month = "1",
day = "1",
doi = "10.1111/j.1399-0039.1995.tb02414.x",
language = "English",
volume = "45",
pages = "49--56",
journal = "HLA",
issn = "2059-2302",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Significance of the HLA‐DQB matching in one‐haplotype identical kidney transplant pairs and the matching analysis by the polymerase chain reaction (PCR) — heteroduplex — polymorphism method

AU - Fukuda, Y.

AU - Kimura, A.

AU - Hoshino, S.

AU - Tashiro, H.

AU - Furukawa, M.

AU - Shintaku, S.

AU - Hori, H.

AU - Sasazuki, Takehiko

AU - Dohi, K.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Abstract: Sixty‐five living related kidney transplant pairs were analyzed for matching at HLA class II loci by the polymerase chain reaction (PCR)‐ sequence specific oligonucleotide probe (SSOP) method. The retrospective HLA matching study revealed that there were many early graft loss cases despite the DQB compatibility, contrary to our expectation. There were 54 DRB1 one‐mismatched cases, in which 7 of the 11 (64%) DQB zero‐mismatched cases had lost their grafts, while the graft loss cases were only 10 of the 43 (23%) DQB one‐mismatched pairs (P value=0.0006). The DQB matching of these cases was studied in detail, because the PCR‐SSOP methods are based on the detection of sequence polymorphisms in a relatively narrow range, i.e., recognized sequences by SSOPs. The PCR ‐ heteroduplex ‐ polymorphism analysis method was developed to analyze the polymorphism in exon 2 of the DQB1 gene. However, all the pairs proved to be compatible for the DQB, demonstrating that the DQB compatibility was associated with a harmful influence on the graft outcome. These observations suggested that the DQB1 incompatibility might exert the low responsiveness to HLA haplo‐identical allogeneic transplants.

AB - Abstract: Sixty‐five living related kidney transplant pairs were analyzed for matching at HLA class II loci by the polymerase chain reaction (PCR)‐ sequence specific oligonucleotide probe (SSOP) method. The retrospective HLA matching study revealed that there were many early graft loss cases despite the DQB compatibility, contrary to our expectation. There were 54 DRB1 one‐mismatched cases, in which 7 of the 11 (64%) DQB zero‐mismatched cases had lost their grafts, while the graft loss cases were only 10 of the 43 (23%) DQB one‐mismatched pairs (P value=0.0006). The DQB matching of these cases was studied in detail, because the PCR‐SSOP methods are based on the detection of sequence polymorphisms in a relatively narrow range, i.e., recognized sequences by SSOPs. The PCR ‐ heteroduplex ‐ polymorphism analysis method was developed to analyze the polymorphism in exon 2 of the DQB1 gene. However, all the pairs proved to be compatible for the DQB, demonstrating that the DQB compatibility was associated with a harmful influence on the graft outcome. These observations suggested that the DQB1 incompatibility might exert the low responsiveness to HLA haplo‐identical allogeneic transplants.

UR - http://www.scopus.com/inward/record.url?scp=0028868089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028868089&partnerID=8YFLogxK

U2 - 10.1111/j.1399-0039.1995.tb02414.x

DO - 10.1111/j.1399-0039.1995.tb02414.x

M3 - Article

VL - 45

SP - 49

EP - 56

JO - HLA

JF - HLA

SN - 2059-2302

IS - 1

ER -