Silkworm midgut proteins interacting with a hemolymph protease inhibitor, CI-8

Yoshinori Ueno, Ningjia He, Minoru Ujita, Kohji Yamamoto, Yutaka Banno, Hiroshi Fujii, Yoichi Aso

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

CI-8 is the chymotrypsin inhibitor in hemolymph from the silkworm, Bombyx mori. It occurs in the midgut at the spinning stage of larva, but little information on the mechanism of its uptake in the midgut is available. We found that two polypeptides interacting with CI-8 are in the midgut membrane, and we purified them using a biotinylated CI-8, viz., p29 and p60, having molecular sizes of 29 kDa and 60 kDa respectively. The structures of p29 and p60 were examined by N-terminal amino acid sequencing and peptide mass mapping, including tryptic digestion. p29 was highly similar to the matured 19G1-30K lipoprotein from hemolymph, but p60 was novel. Purified p29 was recognized by anti-19G1-30K antibody, and was confirmed to be similar to 19G1-30K. The antibody also neutralized the CI-8 binding ability of p29 in the midgut membrane. p29 and p60 are perhaps proteinaceous factors involved in the uptake of CI-8 into the midgut through the membrane.

元の言語英語
ページ(範囲)1557-1563
ページ数7
ジャーナルBioscience, Biotechnology and Biochemistry
70
発行部数7
DOI
出版物ステータス出版済み - 8 1 2006

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Bombyx
Hemolymph
Protease Inhibitors
Membranes
Peptides
Proteins
Peptide Mapping
Antibodies
Protein Sequence Analysis
Chymotrypsin
Larva
Digestion
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

Silkworm midgut proteins interacting with a hemolymph protease inhibitor, CI-8. / Ueno, Yoshinori; He, Ningjia; Ujita, Minoru; Yamamoto, Kohji; Banno, Yutaka; Fujii, Hiroshi; Aso, Yoichi.

:: Bioscience, Biotechnology and Biochemistry, 巻 70, 番号 7, 01.08.2006, p. 1557-1563.

研究成果: ジャーナルへの寄稿記事

Ueno, Yoshinori ; He, Ningjia ; Ujita, Minoru ; Yamamoto, Kohji ; Banno, Yutaka ; Fujii, Hiroshi ; Aso, Yoichi. / Silkworm midgut proteins interacting with a hemolymph protease inhibitor, CI-8. :: Bioscience, Biotechnology and Biochemistry. 2006 ; 巻 70, 番号 7. pp. 1557-1563.
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AU - Banno, Yutaka

AU - Fujii, Hiroshi

AU - Aso, Yoichi

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