Simulated microgravity culture system for a 3-D carcinoma tissue model

K. Nakamura, H. Kuga, T. Morisaki, Eishi Baba, N. Sato, Kazuhiro Mizumoto, K. Sueishi, M. Tanaka, Mitsuo Katano

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

An in vitro organotypic culture model is needed to understand the complexities of carcinoma tissue consisting of carcinoma cells, stromal cells, and extracellular matrices. We developed a new in vitro model of carcinoma tissue using a rotary cell culture system with four disposable vessels (RCCS™-4D) that provides a simulated microgravity condition. Solid collagen gels containing human pancreatic carcinoma NOR-P1 cells and fibroblasts or minced human pancreatic carcinoma tissue were cultured under a simulated microgravity condition or a static 1g condition for seven days. NOR-P1 cultures subjected to the simulated microgravity condition showed greater numbers of mitotic, cycling (Ki-67-positive), nuclear factor-κ B-activating cells, and a lower number of apoptotic cells than were shown by cultures subjected to the static 1g condition. In addition, human pancreatic carcinoma specimens cultured under the simulated microgravity condition maintained the heterogeneous composition and cellular activity (determined by the cycling cell ratio and mitotic index) of the original carcinoma tissue better than static culture conditions. This new 3-D rotary cell culture system with four disposal vessels may be useful for in vitro studies of complex pancreatic carcinoma tissue.

元の言語英語
ページ(範囲)1068-1076
ページ数9
ジャーナルBioTechniques
33
発行部数5
出版物ステータス出版済み - 11 1 2002

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Weightlessness
Microgravity
Cell culture
Tissue
Carcinoma
Cell Culture Techniques
Cells
B-Cell Activating Factor
Mitotic Index
Fibroblasts
Stromal Cells
Extracellular Matrix
Collagen
Cell Count
Gels
Pancreatic Carcinoma
Chemical analysis
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Nakamura, K., Kuga, H., Morisaki, T., Baba, E., Sato, N., Mizumoto, K., ... Katano, M. (2002). Simulated microgravity culture system for a 3-D carcinoma tissue model. BioTechniques, 33(5), 1068-1076.

Simulated microgravity culture system for a 3-D carcinoma tissue model. / Nakamura, K.; Kuga, H.; Morisaki, T.; Baba, Eishi; Sato, N.; Mizumoto, Kazuhiro; Sueishi, K.; Tanaka, M.; Katano, Mitsuo.

:: BioTechniques, 巻 33, 番号 5, 01.11.2002, p. 1068-1076.

研究成果: ジャーナルへの寄稿記事

Nakamura, K, Kuga, H, Morisaki, T, Baba, E, Sato, N, Mizumoto, K, Sueishi, K, Tanaka, M & Katano, M 2002, 'Simulated microgravity culture system for a 3-D carcinoma tissue model', BioTechniques, 巻. 33, 番号 5, pp. 1068-1076.
Nakamura K, Kuga H, Morisaki T, Baba E, Sato N, Mizumoto K その他. Simulated microgravity culture system for a 3-D carcinoma tissue model. BioTechniques. 2002 11 1;33(5):1068-1076.
Nakamura, K. ; Kuga, H. ; Morisaki, T. ; Baba, Eishi ; Sato, N. ; Mizumoto, Kazuhiro ; Sueishi, K. ; Tanaka, M. ; Katano, Mitsuo. / Simulated microgravity culture system for a 3-D carcinoma tissue model. :: BioTechniques. 2002 ; 巻 33, 番号 5. pp. 1068-1076.
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